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Chemodex
DAF-2 Solution
530
CHF
CHF 530.00
In stock
CDX-D0584-M0011 mgCHF 530.00
| Product Details | |
|---|---|
| Synonyms | 4,5-Diaminofluorescein |
| Product Type | Chemical |
| Properties | |
| Formula | C20H14N2O5 |
| MW | 362.34 |
| CAS | 205391-01-1 |
| Source/Host Chemicals | Synthetic. |
| Purity Chemicals | ≥95% (HPLC) |
| Appearance | Liquid. |
| Solubility | Soluble in DMSO. |
| Concentration | 5mM in DMSO (1mg in 0.55ml DMSO) |
| Identity | Determined by 1H-NMR. |
| Declaration | Manufactured by Chemodex. |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| InChi Key | LTYUPYUWXRTNFQ-UHFFFAOYSA-N |
| Smiles | NC1=CC2=C(C=C1N)C1(OC2=O)C2=CC=C(O)C=C2OC2=C1C=CC(O)=C2 |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
Keep cool and dry. Protect from light. |
| Use/Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
DAF-2 is highly sensitive reagent for NO detection and determination of nitric oxide synthase activity. DAF-2, however, remains essentially nonfluorescent until it reacts with the nitrosonium cation (produced by spontaneous oxidation of nitric oxide) to form a fluorescent heterocycle, which becomes trapped in the cell's cytoplasm. This sensitive fluorescent probe has been used to identify individual nitric oxide-producing neurons in brain slices, in mitochondria and in living plant cells. Spectral data: λex 505nm; λem 518nm in 0.1M Tris pH8.0.
Product References
(1) M. Kirsch et al.; J. Pineal Res. 40(1), 10 (2006) | (2) V.M. Dirsch et al.; Biol. Proc. Online 5(1), 136 (2003) | (3) N. Nagata et al.; J. Biochem. 125(4), 658 (1999) | (4) H. Kojima et al.; Chem. Pharm. Bull. 46, 373 (1998) | (5) H. Kojima et al.; Anal. Chem. 70, 2446 (1998)
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