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Chemodex
Fluorescamine
58
CHF
CHF 58.00
In stock
CDX-F0003-M100100 mgCHF 58.00
CDX-F0003-M250250 mgCHF 110.00
CDX-F0003-G0011 gCHF 361.00
| Product Details | |
|---|---|
| Synonyms | 1'(3'H)-isobenzofuran]-3,3'-dione, 4-phenyl-spiro[furan-2(3H); 4-Phenylspiro[furan-2(3H),1'-phthalan]-3,3'-dione; Fluram |
| Product Type | Chemical |
| Properties | |
| Formula | C17H10O4 |
| MW | 278.26 |
| CAS | 38183-12-9 |
| Source/Host Chemicals | Synthetic |
| Purity Chemicals | ≥98% (TLC) |
| Appearance | Off-white to yellow powder. |
| Solubility | Soluble in acetone (50 mg/ml), water (partly), ethanol, chloroform, DMSO or acetonitrile. |
| Identity | Determined by NMR. |
| Declaration | Manufactured by Chemodex. |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| InChi Key | ZFKJVJIDPQDDFY-UHFFFAOYSA-N |
| Smiles | O=C1OC2(OC=C(C2=O)C2=CC=CC=C2)C2=CC=CC=C12 |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | +20°C |
| Long Term Storage | +20°C |
| Handling Advice |
Keep cool and dry. Protect from light and moisture. |
| Use/Stability | Stable for at least 2 years after receipt when stored at +20°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
A non-fluorescent reagent that reacts readily under mild conditions with primary amino groups in amino acids and peptides to form stable and highly fluorescent derivatives. Low background due to hydrolysis. Useful in fluorometric assays of amino acids, protein, and proteolytic enzymes. Effectively blocks newly generated amino termini in protein sequence analysis by the Beckman automated sequencer. Spectral data: Exc. 390nm/Em. 475 nm in 0.5 M borate buffer, pH 8.5, after derivatization with L-leucine.
Product References
(1) S. Udenfriend, et al.; Science 178, 871 (1972) | (2) M. Weigele, et al.; JACS 94, 5927 (1972) | (3) S. Stein, et al.; Arch. Biochem. Biophys. 155, 202 (1973) | (4) P. Bohlen, et al.; Arch. Biochem. Biophys. 163, 390 (1974) | (5) C.Y. Lai, et al.; Meth. Enzymol. 47, 236 (1977) | (6) K. Sogowa & K.J. Takahaski; Biochem. 83, 1783 (1978) | (7) A.S. Bhown, et al.; Anal. Biochem. 112, 158 (1981)