AdipoGen Life Sciences

anti-Sirtuin 6 (human), pAb

CHF 190.00

In stock

AG-25A-0101-C100100 µgCHF 190.00

Figure 1: Western blot analysis using anti-Sirtuin 6 (human), pAb (Prod. No. AG-25A-0101) at 1:2'000 dilution.
1: Human Sirtuin 6 (intact form) (His-tagged).
2: Human Sirtuin 1 (His-tagged).
3: Human Sirtuin 2 (His-tagged).
4: Human Sirtuin 5 (His-tagged).
5: Mouse IDO (His-tagged) (negative control).

Specifications / Handling

More Information
Product Details
Synonyms	SIRT6; ; NAD-dependent Deacetylase Sirtuin-6; Mono-ADP-ribosyltransferase Sirtuin 6; SIR2-like Protein 6; SIR2L6
Product Type	Polyclonal Antibody
Properties
Source/Host	Rabbit
Immunogen/Antigen	Recombinant human sirtuin 6.
Application	ELISA: (direct and indirect: 1:2’000-1:5’000) Western Blot: (1:2’000-1:5’000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application. Note: Tested on recombinant proteins and/or target-protein transfected cell lines in ELISA, Western Blot and/or FACS.
Crossreactivity	Human
Specificity	Recognizes human sirtuin 6. Detects a band of ~42kDa by Western blot.
Purity Detail	Protein A-affinity purified.
Concentration	1mg/ml
Formulation	Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Isotype Negative Control	Rabbit IgG
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 6 months after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Sirtuin 6 (SIRT6) is a NAD-dependent protein deacetylase with activity towards 'Lys-9' and 'Lys-56' of histone H3. It modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. It is required for genomic stability and for normal IGF1 serum levels and normal glucose homeostasis. It also regulates cellular senescence and apoptosis. Unlike most sirtuins, SIRT6 preferentially removes long-chain fatty-acyl, rather than acetyl, protein modifications. This activity regulates secretion of the inflammation-associated protein TNF-α.

Call	+41 61 926 6040
Fax	+41 61 926 6049