AdipoGen Life Sciences

VISTA [B7-H5] (mouse):Fc (human) (rec.)

CHF 335.00

In stock

AG-40B-0164-C05050 µgCHF 335.00

Figure 1: Recombinant VISTA [B7-H5] (mouse):Fc (human) (rec.) (Prod. No. AG-40B-0164) inhibits anti-CD3/CD28 antibody-induced proliferation of mouse CD4+ T lymphocytes. Method: CD4+ T cells from C57BL/6 mice are activated with coated anti-CD3/CD28 and VISTA (mouse):Fc or a control Fc (Prod. No. AG-35B-0007) (5 µg/ml). Plots illustrate the percentage of dividing cells by using anti-CD44 and the dye Cell Trace Violet (CTV).
Picture Courtesy of G. Guarda and A. Zenobi, Institute for Research in Biomedicine, Bellinzona (Switzerland).

Specifications / Handling

More Information
Product Details
Synonyms	V-type Immunoglobulin Domain-containing Suppressor of T Cell Activation; Platelet Receptor Gi24; Stress-induced Secreted Protein-1; Sisp-1; V-set Domain-containing Immunoregulatory Receptor
Product Type	Protein
Properties
Source/Host	HEK 293 cells
Sequence	The extracellular domain of mouse VISTA (aa 33-191) is fused at the C-terminus to the Fc portion of human IgG1.
Crossreactivity	Mouse
MW	~65-70kDa (SDS-PAGE)
Purity	≥95% (SDS-PAGE)
Endotoxin Content	<0.01EU/μg purified protein (LAL test).
Concentration	1mg/ml after reconstitution.
Reconstitution	Reconstitute with 50μl sterile water.
Formulation	Lyophilized. Contains PBS.
Protein Negative Control	Fc (human) IgG1 Control (rec.)
Other Product Data	UniProt link Q9D659: VISTA (mouse)
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability	Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Mouse VISTA is a 55- to 65kDa type I Ig membrane protein with the extracellular domain homologous to PD-L1. VISTA is mainly expressed on hematopoietic tissues (spleen, thymus and bone marrow) and on myeloid cells with lower expression on CD4+ and CD8+ T cells. VISTA is a new negative checkpoint regulator that potently suppresses T cell activation. Overexpression of VISTA on tumors inhibits the protective anti-tumor immunity and blockade of VISTA enhances antitumor immunity in multiple tumor models. VISTA on APC delivers negative signaling to T cells via a yet-to-identify binding partner on T cells.

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