AdipoGen Life Sciences

VISTA [B7-H5] (human):Fc (human) (rec.)

CHF 335.00
In stock
AG-40B-0163-C05050 µgCHF 335.00
More Information
Product Details
Synonyms V-type Immunoglobulin Domain-containing Suppressor of T Cell Activation; Platelet Receptor Gi24; Stress-induced Secreted Protein-1; Sisp-1; V-set Domain-containing Immunoregulatory Receptor
Product Type Protein
Properties
Source/Host HEK 293 cells
Sequence

The extracellular domain of human VISTA (aa 33-194) is fused at the C-terminus to the Fc portion of human IgG1.

Crossreactivity Human
Mouse
MW ~65-70kDa (SDS-PAGE)
Purity ≥95% (SDS-PAGE)
Endotoxin Content <0.01EU/μg purified protein (LAL test).
Concentration 1mg/ml after reconstitution.
Reconstitution Reconstitute with 50μl sterile water.
Formulation Lyophilized. Contains PBS.
Protein Negative Control

Fc (human) IgG1 Control (rec.)

Other Product Data

UniProt link Q9H7M9: VISTA (human)

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

Human VISTA is a 55- to 65kDa type I Ig membrane protein with the extracellular domain homologous to PD-L1. VISTA is mainly expressed on hematopoietic tissues (spleen, thymus and bone marrow) and on myeloid cells with lower expression on CD4+ and CD8+ T cells. VISTA is a new negative checkpoint regulator that potently suppresses T cell activation. Overexpression of VISTA on tumors inhibits the protective anti-tumor immunity and blockade of VISTA enhances antitumor immunity in multiple tumor models. VISTA on APC delivers negative signaling to T cells via a yet-to-identify binding partner on T cells.

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