FAQ: Biochemicals (Small Molecules)
Many of the AdipoGen biochemicals are sold in very small quantities. Some products are transparent and/or are lyophilized to thin films on the walls of the vial making it difficult to see the contents of the vial. Add an appropriate solvent (as indicated on the product datasheet) and vortex the vial briefly or pipette the solvent on the walls of the vial to insure that all of the compound is recovered.
How should I dissolve the biochemical which I purchased from you?
The product datasheet includes information on the compound’s solubility. Many compounds that are not directly soluble in water can be dissolved in alcohols, or solvents such as DMSO or DMF to create a concentrated stock solution. These stock solutions are often reasonably stable for storage, and can be diluted to the desired final treatment concentration with water or aqueous media at the time of use. We strongly recommend using anhydrous solvents whenever working with a compound that is not water soluble.
The biochemical does not dissolve at the indicated concentration. What should I do?
In most cases the speed at which a crystalline solid will dissolve is dependent upon three main factors, the affinity of the compound for the solvent in question, the size of the crystals within the sample, and the temperature. Warming of the solution is often the easiest way to speed up solubilization.
My product arrived at room temperature, but the label says it should be stored at -20°C. Is the product still good?
The storage temperature indicated on the vial is the optimal condition for long term storage. Shipping conditions might vary and are listed on the product datasheet. However, many compounds are quite stable at warmer temperatures for short periods of time. Contact our Technical Support if you have concers.
The datasheet that came with my product does not state that the material is sterile. Will it contaminate my cells?
Please note that all AdipoGen products are not sterile unless otherwise indicated on the product datasheet. We cannot guarantee that it will not contaminate your cells. However there are a few basic precautions that can easily prevent bacterial or fungal contamination of your cell culture. First, make sure that the culture media contains an antibiotic; this alone will prevent the majority of contamination issues. Second, dissolve the product in either 100% ethanol or 100% DMSO. These solvents are not conducive to bacterial or fungal growth, and using them will prevent contaminants from multiplying in your stock solution. Finally, most small molecules can be sterile filtered through a 0.22 µm filter if desired. Please note however that there will always be some loss of the product to the filter.
Can I use the AdipoGen small molecules as analytical standards and/or calibrants?
All AdipoGen small molecules are research reagents only. If not otherwise indicated AdipoGen does not offer biochemicals that may be used as analytical standard or calibrant. Purity, analytical techniques and precision of packaging of our biochemicals do not comply with the requirements needed for such application.
Can I use AdipoGen small molecules for human applications?
All AdipoGen reagents are not for human application, and are not produced under GMP conditions as requested for pharmaceuticals.