AMC Standard Solution

Specifications / Handling

More Information
Product Details
Synonyms	7-Amino-4-methylcoumarin; Coumarin 120
Product Type	Chemical
Properties
Formula	C₁₀H₉NO₂
MW	175.2
CAS	26093-31-2
Appearance	Liquid.
Formulation	50µM stock solution in DMSO.
Declaration	Manufactured by Boston Biochem
Other Product Data	Use: Serial dilute in reaction buffer to form a standard curve within the range of sensitivity determined for the assay and plate reader. The recommend upper final concentration for AMC curves is 500 nM. The excitation and emission wavelengths of AMC are 360nm and 460nm, respectively. Other wavelengths may be used as long as measurements are consistent for both the experimental samples and the dilution curve.
InChi Key	GLNDAGDHSLMOKX-UHFFFAOYSA-N
Smiles	CC1=CC(=O)OC2=C1C=CC(N)=C2
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	-20°C
Long Term Storage	-20°C
Handling Advice	Avoid freeze/thaw cycles. Protect from light.
Use/Stability	Stable for at least 1 year after receipt when stored at -20°C. Store solutions at -20°C in the dark.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

A fluorogenic standard useful for quantitating assays monitoring amido-4-methylcoumarin (AMC) release. These include ubiquitin-AMC, SUMO-1-AMC, NEDD8-AMC, ISG15-AMC and proteasome substrates. The concentration of the AMC released can be calculated from a standard curve prepared with AMC standards.

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