AFC Standard Solution
|Formulation||50µM stock solution in DMSO.|
|Declaration||Manufactured by Boston Biochem|
|Other Product Data||
Use: Serial dilute in reaction buffer to form a standard curve within the range of sensitivity determined for the assay and plate reader. The recommend upper final concentration for AFC curves is 500 nM. The excitation and emission wavelengths of AFC are 400nm and 505nm, respectively.
|Shipping and Handling|
|Short Term Storage||-20°C|
|Long Term Storage||-20°C|
Avoid freeze/thaw cycles.
Protect from light.
Stable for at least 1 year after receipt when stored at -20°C.
Store solutions at -20°C in the dark.
|Product Specification Sheet|
A fluorogenic standard useful for quantitating assays monitoring 7-amino-4-trifluoromethylcoumarin (AFC) release such as those utilizing ubiquitin-AFC. The concentration of the AFC released can be calculated from a standard curve prepared with AFC standards.