AFC Standard Solution

CHF 64.00
In stock
AG-CS1-0104-R100100 µlCHF 64.00
More Information
Product Details
Synonyms 7-Amino-4-trifluoromethylcoumarin
Product Type Chemical
Properties
Formula

C10H6F3NO2

MW 229.2
CAS 53518-15-3
Appearance Liquid.
Formulation 50µM stock solution in DMSO.
Declaration Manufactured by Boston Biochem
Other Product Data

Use: Serial dilute in reaction buffer to form a standard curve within the range of sensitivity determined for the assay and plate reader. The recommend upper final concentration for AFC curves is 500 nM. The excitation and emission wavelengths of AFC are 400nm and 505nm, respectively.

InChi Key JBNOVHJXQSHGRL-UHFFFAOYSA-N
Smiles NC1=CC=C2C(OC(=O)C=C2C(F)(F)F)=C1
Shipping and Handling
Shipping BLUE ICE
Short Term Storage -20°C
Long Term Storage -20°C
Handling Advice Avoid freeze/thaw cycles.
Protect from light.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Store solutions at -20°C in the dark.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

A fluorogenic standard useful for quantitating assays monitoring 7-amino-4-trifluoromethylcoumarin (AFC) release such as those utilizing ubiquitin-AFC. The concentration of the AFC released can be calculated from a standard curve prepared with AFC standards.

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