AMC Standard Solution
|Synonyms||7-Amino-4-methylcoumarin; Coumarin 120|
|Formulation||50µM stock solution in DMSO.|
|Declaration||Manufactured by Boston Biochem|
|Other Product Data||
Use: Serial dilute in reaction buffer to form a standard curve within the range of sensitivity determined for the assay and plate reader. The recommend upper final concentration for AMC curves is 500 nM. The excitation and emission wavelengths of AMC are 360nm and 460nm, respectively. Other wavelengths may be used as long as measurements are consistent for both the experimental samples and the dilution curve.
|Shipping and Handling|
|Short Term Storage||-20°C|
|Long Term Storage||-20°C|
Avoid freeze/thaw cycles.
Protect from light.
Stable for at least 1 year after receipt when stored at -20°C.
Store solutions at -20°C in the dark.
|Product Specification Sheet|
A fluorogenic standard useful for quantitating assays monitoring amido-4-methylcoumarin (AMC) release. These include ubiquitin-AMC, SUMO-1-AMC, NEDD8-AMC, ISG15-AMC and proteasome substrates. The concentration of the AMC released can be calculated from a standard curve prepared with AMC standards.