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Chemodex
5-Dodecanoylaminofluorescein
122
CHF
CHF 122.00
In stock
CDX-D0162-M05050 mgCHF 122.00
CDX-D0162-M250250 mgCHF 489.00
| Product Details | |
|---|---|
| Synonyms | AFC12; DAF; 5-(N-Dodecanoyl)aminofluorescein |
| Product Type | Chemical |
| Properties | |
| Formula | C32H35NO6 |
| MW | 529.62 |
| CAS | 107827-77-0 |
| Source/Host Chemicals | Synthetic. |
| Purity Chemicals | ≥99% |
| Appearance | Yellow to beige powder. |
| Solubility | Soluble in DMF or DMSO. |
| Identity | Determined by NMR. |
| Declaration | Manufactured by Chemodex. |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| InChi Key | VJNRUQFWKRLMSL-UHFFFAOYSA-N |
| Smiles | CCCCCCCCCCCC(=O)NC1=CC2=C(C=C1)C1(OC2=O)C2=CC=C(O)C=C2OC2=C1C=CC(O)=C2 |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | +20°C |
| Long Term Storage | +4°C |
| Handling Advice |
Keep cool and dry. Protect from light and moisture. |
| Use/Stability | Stable for at least 2 years after receipt when stored at +4°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Lipophilic fluorescent cell staining probe contains a C12 alkyl chain that will bind to membranes with the fluorophore at the aqueous interface and the alkyl tail protruding into the lipid interior. Useful to label plasma membranes. Binds strongly to amphiphilic block copolymer micelles. Useful for determining the critical micelle concentration (CMC) of various detergents based on fluorescence polarization (anisotropy). Lipophilic fluorescein probes are commonly utilized for fluorescence recovery after photobleaching (FRAP) measurements of lipid lateral diffusion. Used for visualization of proteins in 1-D and 2-D SDS-PAGE gels. Spectral data: λex=490nm, λem=520nm.
Product References
(1) D. Beer Stolz, et al.; BBRC 184, 160 (1992) | (2) M. Stepanek & K. Prochazka; Langmuir 15, 8800 (1999) | (3) C. Kang, et al.; 24, 3297 (2003) | (4) M.D. Wenger, et al.; Anal. Biochem. 327, 48 (2005) | (5) M.V. Thorsteinsson, et al.; Anal. Biochem. 340, 220 (2005)
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