Nile Red

CHF 191.00
In stock
CDX-N0107-G0011 gCHF 191.00
CDX-N0107-G0055 gCHF 766.00
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Product Details
Synonyms Nile Blue A Oxazone; Phenoxazone 9; 9-(Diethylamino)-5H-benzo[a]phenoxazin-5-one
Product Type Chemical
Properties
Formula

C20H18N2O2

MW 318.4
CAS 7385-67-3
Source/Host Chemicals Synthetic.
Purity Chemicals ≥98% (HPLC)
Appearance Green to brown powder.
Solubility Soluble in chloroform.
Identity Determined by NMR.
Declaration Manufactured by Chemodex.
Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

InChi Key VOFUROIFQGPCGE-UHFFFAOYSA-N
Smiles CCN(CC)C1=CC=C2N=C3C(OC2=C1)=CC(=O)C1=C3C=CC=C1
Shipping and Handling
Shipping AMBIENT
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Keep cool and dry.
Protect from light and moisture.
Use/Stability Stable for at least 2 years after receipt when stored at -20°C.
Documents
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF

Uncharged, hydrophobic, photostable fluorescent probe that strongly fluoresces bright red in hydrophobic (lipid-rich) environments, but is almost nonfluorescent in water. This lipophilic stain is commonly used for the detection of intracellular lipid droplets in cells such as adipocytes by fluorescence microscopy and flow cytometry. It has been shown to detect the exposure or formation of new hydrophobic surfaces induced by ligand binding to calmodulin, oligomerization of melittin, or unfolding of ovalbumin during early thermal denaturation. This product has been employed to stain cell structures, as well as to visualize and localize colloidal drug carriers. Has been used to stain lipophilic proteins that have been separated by SDS-PAGE electrophoresis or to detect sphingolipids on thin-layer chromatograms. The fluorescence is unaffected between pH 4.5 and 8.5 and may be used in dilute concentrations to investigate hydrophobic protein sites. Spectral properties: Intracellular fat vacuoles, filled with neutral lipids such as cholesterol, lipoproteins and triglycerides will fluoresce green (Abs/Em =485/525) while polar lipids, such as phospholipids, will fluoresce red (Abs/Em = 552/636 nm). Extinction coeff. 0.60 ± 0.05 (0.01g/l; 0,5cm).

Product References

(1) P. Greenspan, et al.; J. Cell Biol. 100, 965 (1985) | (2) S.D. Fowler & P. Greenspan; J. Histochem. Cytochem. 33, 833 (1985) | (3) D.L. Sackett & J. Wolff; Anal. Biochem. 167, 228 (1987) | (4) F.J. Alba, et al; Biotechniques 21, 625 (1996) | (5) A. Lamprecht, et al.; J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 787, 415 (2003) | (6) A. Makino, et al.; Biochem. 45, 4530 (2006) | (7) T. Werner, et al.; Cytometry A 69, 200 (2006) | (8) B.W. Halstead, et al.; J. Appl. Toxicol. 26, 169 (2006) | (9) R. Varghese, et al.; Chemistry 16, 9040 (2010)

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