|Synonyms||Nile Blue A Oxazone; Phenoxazone 9; 9-(Diethylamino)-5H-benzo[a]phenoxazin-5-one|
|Purity Chemicals||≥98% (HPLC)|
|Appearance||Green to brown powder.|
|Solubility||Soluble in chloroform.|
|Identity||Determined by NMR.|
|Declaration||Manufactured by Chemodex.|
|Other Product Data||
Click here for Original Manufacturer Product Datasheet
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
Keep cool and dry.
Protect from light and moisture.
|Use/Stability||Stable for at least 2 years after receipt when stored at -20°C.|
|Product Specification Sheet|
Uncharged, hydrophobic, photostable fluorescent probe that strongly fluoresces bright red in hydrophobic (lipid-rich) environments, but is almost nonfluorescent in water. This lipophilic stain is commonly used for the detection of intracellular lipid droplets in cells such as adipocytes by fluorescence microscopy and flow cytometry. It has been shown to detect the exposure or formation of new hydrophobic surfaces induced by ligand binding to calmodulin, oligomerization of melittin, or unfolding of ovalbumin during early thermal denaturation. This product has been employed to stain cell structures, as well as to visualize and localize colloidal drug carriers. Has been used to stain lipophilic proteins that have been separated by SDS-PAGE electrophoresis or to detect sphingolipids on thin-layer chromatograms. The fluorescence is unaffected between pH 4.5 and 8.5 and may be used in dilute concentrations to investigate hydrophobic protein sites. Spectral properties: Intracellular fat vacuoles, filled with neutral lipids such as cholesterol, lipoproteins and triglycerides will fluoresce green (Abs/Em =485/525) while polar lipids, such as phospholipids, will fluoresce red (Abs/Em = 552/636 nm). Extinction coeff. 0.60 ± 0.05 (0.01g/l; 0,5cm).
(1) P. Greenspan, et al.; J. Cell Biol. 100, 965 (1985) | (2) S.D. Fowler & P. Greenspan; J. Histochem. Cytochem. 33, 833 (1985) | (3) D.L. Sackett & J. Wolff; Anal. Biochem. 167, 228 (1987) | (4) F.J. Alba, et al; Biotechniques 21, 625 (1996) | (5) A. Lamprecht, et al.; J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 787, 415 (2003) | (6) A. Makino, et al.; Biochem. 45, 4530 (2006) | (7) T. Werner, et al.; Cytometry A 69, 200 (2006) | (8) B.W. Halstead, et al.; J. Appl. Toxicol. 26, 169 (2006) | (9) R. Varghese, et al.; Chemistry 16, 9040 (2010)