5-Dodecanoylaminofluorescein

Specifications / Handling

More Information
Product Details
Synonyms	AFC12; DAF; 5-(N-Dodecanoyl)aminofluorescein
Product Type	Chemical
Properties
Formula	C₃₂H₃₅NO₆
MW	529.62
CAS	107827-77-0
Source/Host Chemicals	Synthetic.
Purity Chemicals	≥99%
Appearance	Yellow to beige powder.
Solubility	Soluble in DMF or DMSO.
Identity	Determined by NMR.
Declaration	Manufactured by Chemodex.
Other Product Data	Click here for Original Manufacturer Product Datasheet Our product description may differ slightly from the original manufacturers product datasheet.
InChi Key	VJNRUQFWKRLMSL-UHFFFAOYSA-N
Smiles	CCCCCCCCCCCC(=O)NC1=CC2=C(C=C1)C1(OC2=O)C2=CC=C(O)C=C2OC2=C1C=CC(O)=C2
Shipping and Handling
Shipping	AMBIENT
Short Term Storage	+20°C
Long Term Storage	+4°C
Handling Advice	Keep cool and dry. Protect from light and moisture.
Use/Stability	Stable for at least 2 years after receipt when stored at +4°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Lipophilic fluorescent cell staining probe contains a C12 alkyl chain that will bind to membranes with the fluorophore at the aqueous interface and the alkyl tail protruding into the lipid interior. Useful to label plasma membranes. Binds strongly to amphiphilic block copolymer micelles. Useful for determining the critical micelle concentration (CMC) of various detergents based on fluorescence polarization (anisotropy). Lipophilic fluorescein probes are commonly utilized for fluorescence recovery after photobleaching (FRAP) measurements of lipid lateral diffusion. Used for visualization of proteins in 1-D and 2-D SDS-PAGE gels. Spectral data: λex=490nm, λem=520nm.

Product References

(1) D. Beer Stolz, et al.; BBRC 184, 160 (1992) | (2) M. Stepanek & K. Prochazka; Langmuir 15, 8800 (1999) | (3) C. Kang, et al.; 24, 3297 (2003) | (4) M.D. Wenger, et al.; Anal. Biochem. 327, 48 (2005) | (5) M.V. Thorsteinsson, et al.; Anal. Biochem. 340, 220 (2005)

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