5(6)-SFX

Specifications / Handling

More Information
Product Details
Synonyms	6-(Fluorescein-5(6)-carboxamido)hexanoicacid N-succinimidyl ester; 5(6)-FAM-X, SE; C6-F-NHS; FHS
Product Type	Chemical
Properties
Formula	C₃₁H₂₆N₂O₁₀
MW	586.6
CAS	114616-31-8
Source/Host Chemicals	Synthetic.
Purity Chemicals	≥75% (HPLC)
Appearance	Yellow to orange powder.
Solubility	Soluble in dimethylformamide or methanol.
Identity	Determined by ¹H-NMR.
Declaration	Manufactured by Chemodex.
Other Product Data	Click here for Original Manufacturer Product Datasheet Our product description may differ slightly from the original manufacturers product datasheet.
InChi Key	AZTAZTWVNFVDPK-UHFFFAOYSA-N
Smiles	O=C(C1=C2C=CC=C1)OC32C4=C(C=C(O)C=C4)OC5=CC(O)=CC=C53.CC(NCCCCCC(ON6C(CCC6=O)=O)=O)=O
Shipping and Handling
Shipping	AMBIENT
Short Term Storage	-20°C
Long Term Storage	-20°C
Handling Advice	Keep cool and dry. Protect from light and moisture.
Use/Stability	Stable for at least 2 years after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Amine-reactive fluorescent probe for introducing a fluorescein label at a nucleophilic residue of a target molecule. This fluorescein labeling reagent has an added (X) spacer to improve accessibility of the reactive group by nucleophiles in preparation of bioconjugates. The reactive N-hydroxysuccinimide (NHS) group can be used for easy attachment to proteins or amine containing nucleotides using simple procedures. Spectral properties: λex 490 nm; λem 514 nm in DMF (pH 8.2) and λex 491 nm; λem 515 nm in 0.1 M Tris pH 9.0.

Product References

(1) G.P. Jamieson, et al.; Cytometry 14, 32 (1993) | (2) K.A. Cruickshank; Anal. Biochem. 269, 21 (1999) | (3) J.J. MacLean, et al.; Biophys. J. 78, 1441 (2000) | (4) W.C. Cooper, et al.; Biophys. J. 78, 1449 (2000) | (5) M.P. Gygi, et al.; Nucleic Acids Res. 30, 2790 (2002)

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