5-SFX

Specifications / Handling

More Information
Product Details
Synonyms	6-(Fluorescein-5-carboxamido)hexanoicacid N-succinimidyl ester; 5-FAM-X, SE
Product Type	Chemical
Properties
Formula	C₃₁H₂₆N₂O₁₀
MW	586.55
CAS	148356-00-7
Source/Host Chemicals	Synthetic.
Purity Chemicals	≥95% (HPLC)
Appearance	Orange powder.
Solubility	Soluble in DMSO, DMF or methanol.
Identity	Determined by NMR.
Declaration	Manufactured by Chemodex.
Other Product Data	Click here for Original Manufacturer Product Datasheet Our product description may differ slightly from the original manufacturers product datasheet.
InChi Key	GQROVDGSBTYULQ-UHFFFAOYSA-N
Smiles	OC1=CC2=C(C(C3=CC=C(C(NCCCCCC(ON4C(CCC4=O)=O)=O)=O)C=C3C(O)=O)=C(C=C5)C(O2)=CC5=O)C=C1
Shipping and Handling
Shipping	AMBIENT
Short Term Storage	-20°C
Long Term Storage	-20°C
Handling Advice	Keep cool and dry. Protect from light and moisture.
Use/Stability	Stable for at least 2 years after receipt when stored at -20°C.
Documents
MSDS	Inquire
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Amine-reactive fluorescent probe for introducing a fluorescein label at a nucleophilic residue of a target molecule. This fluorescein labeling reagent has an added (X) spacer to improve accessibility of the reactive group by nucleophiles in preparation of bioconjugates. The reactive N-hydroxysuccinimide (NHS) group can be used for easy attachment to proteins or amine containing nucleotides using simple procedures. Spectral properties: λex 490nm; λem 514nm in DMF (pH 8.2) and λex 491nm; λem 515nm in 0.1 M Tris pH 9.0.

Product References

(1) G.P. Jamieson, et al.; Cytometry 14, 32 (1993) | (2) K.A. Cruickshank; Anal. Biochem. 269, 21 (1999) | (3) J.J. MacLean, et al.; Biophys. J. 78, 1441 (2000) | (4) W.C. Cooper, et al.; Biophys. J. 78, 1449 (2000) | (5) M.P. Gygi, et al.; Nucleic Acids Res. 30, 2790 (2002)

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