AdipoGen Life Sciences

anti-ACE2 (human), mAb (AC384)

CHF 310.00

In stock

AG-20A-0037-C05050 µgCHF 310.00

AG-20A-0037-C100100 µgCHF 390.00

Figure 1: Western blot analysis using anti-ACE2 (human), mAb (AC384) (Prod. No. AG-20A-0037) at 1:2'000 dilution.
1: Human ACE2 Fc-fusion protein.
2: Human ACE2 (Ecto domain) (FLAG^®-tagged).
3: HepG2 cell lysate.
4: Other Fc-fusion (hGITR-Fc) proteins (negative control).

Specifications / Handling

More Information
Product Details
Synonyms	SARS Receptor; Angiotensin-converting Enzyme 2; ACEH; Metalloprotease MPROT15; SARS-CoV-2 Receptor
Product Type	Monoclonal Antibody
Properties
Clone	AC384
Isotype	Mouse IgG1κ
Immunogen/Antigen	Recombinant human ACE2.
Application	ELISA: (direct or indirect: 1:2'000-1:10'000) Immunofluorescence: (1:100, see reference Stuedle 2023) Western blot: (1:2'000-1:10'000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature). Optimal conditions should be determined individually for each application.
Crossreactivity	Human
Specificity	Recognizes human ACE2.
Purity Detail	Protein G-affinity purified.
Concentration	1mg/ml
Formulation	Liquid. 0.2μm-filtered solution in PBS, pH 7.4.
Isotype Negative Control	Mouse IgG1 Isotype Control
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Angiotensin-converting enzyme2 (ACE2) is an ectoenzyme (carboxypeptidase) with an extracellular catalytic domain that predominantly localizes at the plasma membrane and is thereby able to hydrolyze circulating peptides. ACE2 has approximately 42% sequence identity with ACE, and its cytoplasmic and transmembrane domains show 48% homology to the protein collectrin that plays a critical role in the amino acid absorption of the kidney. ACE2 converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. ACE2 is involved in the regulation of systemic blood pressure and has direct effects on cardiac functions. It is expressed predominantly in endothelial cells of the lung, gut, heart and kidney. ACE2 together with the protease TMPRSS2 acts as a functional receptor for SARS coronavirus as well as for the new highly pathogenic coronavirus, 2019-nCoV/SARS-CoV-2, which is cause for pneumonia COVID-19.

Product References

Development of an enzymatic assay for the detection of neutralizing antibodies against therapeutic angiotensin-converting enzyme 2 (ACE2): K. Liao, et al.; J. Immunol. Meth. 389, 52 (2013)
Role of angiotensin-converting enzyme 2 (ACE2) in diabetic cardiovascular complications: VB. Patel, et al.; Clin. Sci. 126, 471 (2014)
Stapled Peptides Based on Human Angiotensin-Converting Enzyme 2 (ACE2) Potently Inhibit SARS-CoV-2 Infection In Vitro: F. Curreli, et al.; mBio 11, e02451 (2020)
Pomegranate Peel Extract as an Inhibitor of SARS-CoV-2 Spike Binding to Human ACE2 Receptor (in vitro): A Promising Source of Novel Antiviral Drugs: A. Tito, et al.; Front. Chem. 9, 638187 (2021)
Relevance of VEGF and CD147 in different SARS‐CoV‐2 positive digestive tracts characterized by thrombotic damage: D. Bortolotti, et al.; FASEB J. 35, e21969 (2021)
SARS-CoV-2 Spike Protein 1 Activates Microvascular Endothelial Cells and Complement System Leading to Platelet Aggregation: L. Perico, et al.; Front. Immunol. 13, 827146 (2022)
SARS-CoV-2 infects epithelial cells of the blood-cerebrospinal fluid barrier rather than endothelial cells or pericytes of the blood-brain barrier: C. Stuedle, et al.; Fluids and Barriers of the CNS 20, 76 (2023) [Immunofluorescence]

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