anti-ACE2 (human), mAb (AC18F) (Biotin)

CHF 350.00
In stock
AG-20A-0032B-C05050 µgCHF 350.00
More Information
Product Details
Synonyms SARS Receptor; Angiotensin-converting Enzyme 2; ACEH; Metalloprotease MPROT15; SARS-CoV-2 Receptor
Product Type Monoclonal Antibody
Properties
Clone AC18F
Isotype Mouse IgG1κ
Immunogen/Antigen Recombinant human ACE2.
Label/Conjugates Biotin
Application

ELISA: (direct or indirect: 1:2'000-1:10'000)
Flow Cytometry: (1:1'000)
Western blot: (1:2'000-1:10'000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature).
Optimal conditions should be determined individually for each application.

Crossreactivity Human
Specificity

Recognizes human ACE2. Does not detect recombinant human ACE2 that has a tag at the C-terminus of the protein.

Purity Detail Protein G-affinity purified.
Concentration 1mg/ml
Formulation Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Isotype Negative Control

Mouse IgG1 Isotype Control (Biotin)

Accession Number Q9BYF1
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
Protocols Download PDF
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

Angiotensin-converting enzyme2 (ACE2) is an ectoenzyme (carboxypeptidase) with an extracellular catalytic domain that predominantly localizes at the plasma membrane and is thereby able to hydrolyze circulating peptides. ACE2 has approximately 42% sequence identity with ACE, and its cytoplasmic and transmembrane domains show 48% homology to the protein collectrin that plays a critical role in the amino acid absorption of the kidney. ACE2 converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. ACE2 is involved in the regulation of systemic blood pressure and has direct effects on cardiac functions. It is expressed predominantly in endothelial cells of the lung, gut, heart and kidney. ACE2 together with the protease TMPRSS2 acts as a functional receptor for SARS coronavirus as well as for the new highly pathogenic coronavirus, 2019-nCoV/SARS-CoV-2, which is cause for pneumonia COVID-19.

Product References
  1. Co-localization of angiotensin-converting enzyme 2-, octomer-4- and CD34 positive cells in rabbit atherosclerotic plaques: A. Zulli, et al.; Exp. Physiol. 93, 564 (2008)
  2. Role of angiotensin-converting enzyme 2 (ACE2) in diabetic cardiovascular complications: VB. Patel, et al.; Clin. Sci. 126, 471 (2014)
  3. Syncytia formation by SARS-CoV-2-infected cells: J. Buchrieser, et al.; EMBO J. e106267 (2020)
  4. High expression of angiotensin-converting enzyme-2 (ACE2) on tissue macrophages that may be targeted by virus SARS-CoV-2 in COVID-19 patients: X. Song,et al.; bioRxiv (Preprint) (2020)
  5. Distinct immunological signatures discriminate severe COVID-19 from non-SARS-CoV-2-driven critical pneumonia: S. Kreutmair, et al.; Immunity 54, 1578 (2021)
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