Cookie Policy: This site uses cookies to improve your experience. You can find out more about our use of cookies in our Privacy Policy. By continuing to browse this site you agree to our use of cookies.
AdipoGen Life Sciences
ACE2 (human) (rec.)
470
CHF
CHF 470.00
In stock
AG-40B-0192-C05050 µgCHF 470.00
AG-40B-0192-30503 x 50 µgCHF 880.00
Figure 1: ACE2 (human) (rec.) (Prod. No. AG-40B-0192) binds with high affinity to the Spike (RBD) protein of the virus SARS-CoV-2.
Method: ACE2 (human) (rec.) is coated on an ELISA plate at 1μg/ml overnight at 4°C. SARS-CoV-2 Spike Protein S1 (RBD):Fc (human) (rec.) (Prod. No. AG-40B-0194) is added (starting at a concentration of 500ng/ml with a two-fold serial dilution) during one hour at RT and the interaction is then detected using an anti-human IgG (HRP).
Method: ACE2 (human) (rec.) is coated on an ELISA plate at 1μg/ml overnight at 4°C. SARS-CoV-2 Spike Protein S1 (RBD):Fc (human) (rec.) (Prod. No. AG-40B-0194) is added (starting at a concentration of 500ng/ml with a two-fold serial dilution) during one hour at RT and the interaction is then detected using an anti-human IgG (HRP).
| Product Details | |
|---|---|
| Synonyms | SARS Receptor; Angiotensin-converting Enzyme 2; ACEH; Metalloprotease MPROT15; SARS-CoV-2 Receptor |
| Product Type | Protein |
| Properties | |
| Source/Host | HEK 293 cells |
| Sequence |
Extracellular domain of human ACE2 (aa 18-740) are fused at the C-terminus to a FLAG®-tag. |
| Crossreactivity | Human |
| Biological Activity |
Binds to the Spike protein of the coronavirus SARS-CoV-2. |
| MW | ~110kDa (SDS-PAGE); ~240kDa (Dimer, by SEC) |
| Purity | ≥95% (SDS-PAGE) |
| Endotoxin Content | <0.01EU/μg purified protein (LAL test). |
| Concentration | 1mg/ml after reconstitution. |
| Reconstitution | Reconstitute with 50µl endotoxin-free water. |
| Accession Number | Q9BYF1 |
| Formulation | Lyophilized. Contains PBS. |
| Protein Negative Control |
ACE2 (mouse) (rec.) for SARS-CoV-2-binding experiments. |
| Other Product Data |
UniProt link Q9BYF1: ACE2 (human) FLAG is a registered trademark of Sigma-Aldrich Co. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. For maximum product recovery after thawing, centrifuge the vial before opening the cap. |
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Angiotensin-converting enzyme2 (ACE2) is an ectoenzyme (carboxypeptidase) with an extracellular catalytic domain that predominantly localizes at the plasma membrane and is thereby able to hydrolyze circulating peptides. ACE2 has approximately 42% sequence identity with ACE, and its cytoplasmic and transmembrane domains show 48% homology to the protein collectrin that plays a critical role in the amino acid absorption of the kidney. ACE2 converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. ACE2 is involved in the regulation of systemic blood pressure and has direct effects on cardiac functions. It is expressed predominantly in endothelial cells of the lung, gut, heart and kidney. ACE2 together with the protease TMPRSS2 acts as a functional receptor for SARS coronavirus as well as for the new highly pathogenic coronavirus, 2019-nCoV/SARS-CoV-2, which is cause for pneumonia COVID-19.
Product References
- A Multiplex Microsphere IgG Assay for SARS-CoV-2 Using ACE2-Mediated Inhibition as a Surrogate for Neutralization: A. Cameron, et al.; J. Clin. Microbiol. 59, e02489 (2021)
- Simultaneous Measurement of IgM and IgG Antibodies to SARS-CoV-2 Spike, RBD, and Nucleocapsid Multiplexed in a Single Assay on the xMAP INTELLIFLEX DR-SE Flow Analyzer: A. Cameron, et al.; Microbiol. Spectr. e0250721, 2507 (2022)
Related Products
