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Figure 1: Western blot analysis using anti-ACE2 (human), pAb (Prod. No. AG-25A-0042) at 1:2'000 dilution.
1: Human ACE2 Fc-protein.
2: Human ACE2 (ED) (FLAG®-tagged).
ELISA: (direct and indirect: 1:2’000-1:10’000) Western Blot: (1:2’000-1:10’000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
Note:Tested on recombinant proteins and/or target-protein transfected cell lines in ELISA, Western Blot and/or FACS.
Crossreactivity
Human
Specificity
Recognizes human ACE2. Detects a band of ~90kDa by Western blot.
Purity Detail
Protein A-affinity purified.
Concentration
1mg/ml
Formulation
Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Angiotensin-converting enzyme2 (ACE2) is an ectoenzyme (carboxypeptidase) with an extracellular catalytic domain that predominantly localizes at the plasma membrane and is thereby able to hydrolyze circulating peptides. ACE2 has approximately 42% sequence identity with ACE, and its cytoplasmic and transmembrane domains show 48% homology to the protein collectrin that plays a critical role in the amino acid absorption of the kidney. ACE2 converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. ACE2 is involved in the regulation of systemic blood pressure and has direct effects on cardiac functions. It is expressed predominantly in endothelial cells of the lung, gut, heart and kidney. ACE2 together with the protease TMPRSS2 acts as a functional receptor for SARS coronavirus as well as for the new highly pathogenic coronavirus, 2019-nCoV/SARS-CoV-2, which is cause for pneumonia COVID-19.
Product References
Conformational reorganization of the SARS coronavirus spike following receptor binding: implications for membrane fusion: D.R. Beniac, et al.; PLoS One 2, e1082 (2007)
High urinary ACE2 concentrations are associated with severity of glucose intolerance and microalbuminuria: S.E. Park, et al.; Eur. J. Endocrinol. 168, 203 (2013)
Role of angiotensin-converting enzyme 2 (ACE2) in diabetic cardiovascular complications: VB. Patel, et al.; Clin. Sci. 126, 471 (2014)
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