AdipoGen Life Sciences

Nampt (Visfatin/PBEF) (rat) (rec.)

CHF 205.00
In stock
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AG-40A-0058-C05050 µgCHF 470.00
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Product Details
Synonyms Pre-B Cell Colony Enhancing Factor 1; PBEF1; Nicotinamide Phosphoribosyltransferase
Product Type Protein
Properties
Source/Host HEK 293 cells
Sequence Rat Nampt (visfatin/PBEF) (aa 1-491) is fused at the N-terminus to a FLAG®-tag.
Crossreactivity Rat
MW ~60kDa (SDS-PAGE)
Purity ≥90% (SDS-PAGE)
Endotoxin Content <0.1EU/μg purified protein (LAL test; Lonza).
Concentration 0.35mg/ml
Formulation Liquid. 0.2μm-filtered solution in PBS, pH 7.2.
Other Product Data UniProt link Q80Z29: Nicotinamide phosphoribosyltransferase (rat) 
FLAG is a registered trademark of Sigma-Aldrich Co.
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
For maximum product recovery after thawing, centrifuge the vial before opening the cap.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

Nicotinamide phosphoribosyltransferase (Nampt; pre-B cell colony-enhancing factor; PBEF; Visfatin) is an 52kDa adipokine secreted by adipose tissue and involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). Two forms of Nampt exist, an intracellular form (iNampt) and an extracellular form (eNampt). While the function of iNampt as an essential and rate-limiting NAD+ biosynthetic enzyme is well established, the physiological role of eNampt is still a matter of debate. Nampt has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity. Recently NAMPT extracellular (eNAMPT) has been shown to be secreted from macrophages in muscles and to triggers a proliferative signal of muscle stem cell (MusC) by binding to CCR5 located at the surface receptor of MusC.

Product References
  1. The adipocytokine Nampt and its product NMN have no effect on beta-cell survival but potentiate glucose stimulated insulin secretion: R. Spinnler, et al. PLoS One 8, e54106 (2013)
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