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AdipoGen Life Sciences
Nampt (Visfatin/PBEF) (mouse/rat) Dual ELISA Kit
As low as
670
CHF
CHF 670.00
In stock
Only %1 left
AG-45A-0007YEK-KI0196 wellsCHF 670.00
AG-45A-0007YTP-KI012 x 96 wellsCHF 1’220.00
Figure 1: Standard curve
Specifications / Handling
| Product Details | |
|---|---|
| Synonyms | Nicotinamide Phosphoribosyltransferase; PBEF1; Pre-B Cell Colony Enhancing Factor 1 |
| Product Type | Kit |
| Properties | |
| Application Set | Quantitative ELISA |
| Specificity | Detects mouse and rat Nampt. Does not cross-react with human Nampt, mouse adiponectin, mouse resistin, mouse vaspin or mouse RBP4. |
| Crossreactivity |
Mouse Rat |
| Quantity |
1 x 96 wells 2 x 96 wells (Twin Plex) |
| Sensitivity | 50pg/ml |
| Range | 0.5 to 32ng/ml |
| Sample Type | Serum |
| Assay Type | Sandwich |
| Detection Type | Colorimetric |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | +4°C |
| Handling Advice |
After standard reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Plate and reagents should reach room temperature before use. |
| Use/Stability | 18 months after the day of manufacturing. See expiry date on ELISA Kit box. |
| Documents | |
| Manual |
 Download PDF |
| MSDS |
Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Scientific Background Information
Product Description
Nicotinamide phosphoribosyltransferase (NAMPT; Visfatin; PBEF) is a highly conserved protein with important functions in cellular metabolism, inflammation and disease. Intracellular NAMPT (iNAMPT) is the rate-limiting enzyme of the NAD+ salvage pathway and is essential for maintaining cellular NAD+ levels and the activity of NAD+-dependent enzymes. In addition, NAMPT is released into the extracellular space. Extracellular NAMPT (eNAMPT) acts as an adipocytokine and immunometabolic signaling molecule and has been linked to inflammatory, metabolic, vascular and tissue-remodeling processes.
Changes in circulating eNAMPT have been reported in numerous experimental disease models, making NAMPT a valuable biomarker for investigating the interface between metabolism and inflammation. Altered serum NAMPT levels have been associated with obesity, insulin resistance and diabetes, metabolic and fatty liver disease, cardiovascular dysfunction and atherosclerosis, as well as acute and chronic inflammatory conditions.
NAMPT is also increasingly investigated in cancer biology, where altered NAMPT expression and extracellular NAMPT signaling have been linked to metabolic adaptation, tumor-associated inflammation, angiogenesis and immune regulation. Measurement of circulating NAMPT can therefore provide a useful readout in preclinical studies examining metabolic, inflammatory and tumor-associated processes.
The Nampt (Visfatin/PBEF) (mouse/rat) Dual ELISA Kit is designed for the quantitative determination of mouse and rat NAMPT in serum and is particularly suitable for longitudinal biomarker measurements in experimental disease models and intervention studies.
Typical research applications include:
• Obesity, metabolic syndrome, insulin resistance and diabetes models
• Metabolic and fatty liver disease
• Cardiovascular disease and atherosclerosis
• Acute and chronic inflammation
• Sepsis and inflammatory lung injury
• Inflammatory bowel disease and experimental colitis
• Cancer metabolism, tumor-associated inflammation and antitumor immunity
• Aging and NAD+ metabolism
• Monitoring circulating NAMPT during pharmacological, dietary, exercise or other experimental interventions
Product-specific References
- Nampt secreted from cardiomyocytes promotes development of cardiac hypertrophy and adverse ventricular remodeling: V.B. Pillai, et al.; Am. J. Physiol. Heart Circ. Physiol. 304, H415 (2013) [PMC3774498]
- Hepatectomy-related hypophosphatemia: a novel phosphaturic factor in the liver-kidney axis: K. Nomura, et al.; J. Am. Soc. Nephrol. 25, 761 (2013) [PMC3968501]
- Nicotinamide phosphoribosyltransferase inhibitor is a novel therapeutic candidate in murine models of inflammatory lung injury: L. Moreno-Vinasco, et al.; Am. J. Respir. Cell Mol. Biol. 51, 223 (2014) [PMC4148034; Plasma Samples]
- Hepatic NAD+ deficiency as a therapeutic target for non-alcoholic fatty liver disease in ageing: C.-C. Zhou, et al.; Br. J. Pharmacol. 173, 2352 (2016) [PMC4945761]
- NAMPT inhibitor protects ischemic neuronal injury in rat brain via anti-neuroinflammation: C.-X. Chen, et al.; Neuroscience 356, 193 (2017) [PMID: 28528966]
- Nicotinamide phosphoribosyltransferase secreted from microglia via exosome during ischemic injury: Y.-B. Lu, et al.; J. Neurochem. 150, 723 (2019) [PMID: 31269239]
- Neutralization of extracellular NAMPT (nicotinamide phosphoribosyltransferase) ameliorates experimental murine colitis: G. Colombo, et al.; J. Mol. Med. 98, 595 (2020) [PMID: 32338310]
- CD38 downregulation modulates NAD+ and NADP(H) levels in thermogenic adipose tissues: A. Benzi, et al.; BBA Mol. Cell Biol. Lipids 1866, 158819 (2021) [PMID: 33010451]
- NAMPT-targeting PROTAC promotes antitumor immunity via suppressing myeloid-derived suppressor cell expansion: Y. Wu, et al.; Acta Pharm. Sin. B. 12, 2859 (2022) [PMC9214341]
- Extracellular nicotinamide phosphoribosyltransferase boosts IFNg-induced macrophage polarization independently of TLR4: G. Colombo, et al.; iScience 25, 104147 (2022) [PMC8990213]
- Extracellular nicotinamide phosphoribosyltransferase (eNAMPT) neutralization counteracts T cell immune evasion in breast cancer: C. Travelli, et al.; J. Immunother. Cancer 11, e007010 (2023) [PMC10603332]
- Inhibition of ethanol-induced eNAMPT secretion attenuates liver ferroptosis through BAT-Liver communication: Y. Zhou, et al.; Redox Biol. 75, 103274 (2024) [PMC11327441]
- Regulatory roles of NAMPT and NAD+ metabolism in uterine leiomyoma progression: Implications for ECM accumulation, stemness, and microenvironment: Y.-F. Chiang, et al.; Redox Biol. 78, 103411 (2024) [PMC11564007]
- Effects of Nicotinamide Mononucleotide Supplementation and Aerobic Exercise on Metabolic Health and Physical Performance in Aged Mice: Y.-J. Hsu, et al.; Nutrients 17, 3148 (2025) [PMC12525689]
- Extracellular nicotinamide phosphoribosyltransferase (eNAMPT) drives abnormal pericyte-rich vasculature in triple-negative breast cancer: M. Moro, et al.; Angiogenesis 28, 4 (2025) [PMID: 39636369]
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