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AdipoGen Life Sciences
Nampt (Visfatin/PBEF) (human) (IntraCellular) ELISA Kit
As low as
670
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CHF 670.00
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AG-45A-0008YEK-KI0196 wellsCHF 670.00
Figure 1: Standard curve
Specifications / Handling
| Product Details | |
|---|---|
| Synonyms | Nicotinamide Phosphoribosyltransferase; PBEF1; Pre-B Cell Colony Enhancing Factor 1; Intracellular Nampt |
| Product Type | Kit |
| Properties | |
| Application Set | Quantitative ELISA |
| Specificity | Detects human Nampt. Weakly cross-reacts (<15%) with mouse Nampt and rat Nampt. Does not cross-react with human adiponectin, human resistin, human RELM-β or human leptin. |
| Crossreactivity | Human |
| Quantity | 1 x 96 wells |
| Sensitivity | 30pg/ml |
| Range | 0.25 to 16ng/ml |
| Sample Type | Cell Lysate |
| Assay Type | Sandwich |
| Detection Type | Colorimetric |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | +4°C |
| Handling Advice |
After standard reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Plate and reagents should reach room temperature before use. |
| Use/Stability | 12 months after the day of manufacturing. See expiry date on ELISA Kit box. |
| Documents | |
| Manual |
 Download PDF |
| MSDS |
Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Scientific Background Information
Product Description
Nicotinamide phosphoribosyltransferase (NAMPT; Visfatin; PBEF) is a highly conserved enzyme with a central role in cellular NAD+ metabolism. Intracellular NAMPT (iNAMPT) catalyzes the rate-limiting step of the NAD+ salvage pathway, converting nicotinamide to nicotinamide mononucleotide (NMN). Through regulation of the intracellular NAD+ pool, NAMPT influences cellular energy metabolism and the activity of NAD+-dependent enzymes, including sirtuins and PARPs, thereby affecting metabolic adaptation, DNA repair, stress responses, cell survival and inflammatory signaling.
Altered intracellular NAMPT expression and activity have been associated with metabolic disorders, inflammation, aging and cancer. In particular, many cancer cells show increased dependence on NAMPT-mediated NAD+ biosynthesis to maintain the high metabolic and energetic demands required for proliferation and survival. NAMPT has therefore become an important target in cancer metabolism, and NAMPT inhibitors such as FK866 and CHS-828 have been widely used to investigate NAD+ depletion and metabolic vulnerabilities of tumor cells.
Measurement of intracellular NAMPT protein is also useful for studying cellular responses to metabolic stress, inflammatory stimulation, hypoxia, oxidative stress, senescence and pharmacological intervention.
The Nampt (Visfatin/PBEF) (human) (IntraCellular) ELISA Kit is designed for the quantitative determination of human NAMPT protein in cell lysates and provides a convenient tool for investigating changes in intracellular NAMPT levels in cellular and experimental disease models.
Typical research applications include:
• NAD+ salvage pathway and cellular NAD+ metabolism
• Cancer metabolism and metabolic reprogramming
• Evaluation of NAMPT inhibitors and other NAD+-modulating compounds
• Cellular responses to metabolic, oxidative and hypoxic stress
• Inflammation and immune cell activation
• Aging, cellular senescence and longevity research
• Metabolic disease and insulin resistance
• Drug response and resistance mechanisms
• Investigation of NAMPT expression in cellular disease models
The assay measures intracellular NAMPT protein levels and does not directly measure NAMPT enzymatic activity or intracellular NAD+ concentrations.
Product-specific References
- Nicotinamide phosphoribosyltransferase (NAMPT/PBEF/visfatin) is constitutively released from human hepatocytes: A. Garten, et al.; BBRC 391, 376 (2010) [PMID: 19912992]
- Visceral adipose tissue visfatin in nonalcoholic fatty liver disease: R. Gaddipati, et al.; Ann. Hepatol. 9, 266 (2010) [PMID: 20720266]
- Leucocytes are a major source of circulating nicotinamide phosphoribosyltransferase (NAMPT)/pre-B cell colony (PBEF)/visfatin linking obesity and inflammation in humans: D. Friebe, et al.; Diabetologia 54, 1200 (2011) [PMC3071946]
- In vivo Suppression of Visfatin by Oral Glucose Uptake: Evidence for a Novel Incretin-Like Effect by Glucagon-Like Peptide-1 (GLP-1): M. Bala, et al.; J. Clin. Endocrinol. Metab. 96, 2493 (2011) [PMID: 21677044]
- Nicotinamide improves glucose metabolism and affects the hepatic NAD-sirtuin pathway in a rodent model of obesity and type 2 Diabetes: S.J. Yang, et al.; J. Nutritional Biochem. 25, 66 (2014) [PMID: 24314867]
- Resveratrol ameliorates hepatic metaflammation and inhibits NLRP3 inflammasome activation: S.J. Yang & Y. Lim; Matabolism 63, 693 (2014) [PMID: 24629563]
- CTRP13 Mitigates Abdominal Aortic Aneurysm Formation via NAMPT1: W. Xu, et al.; Mol. Ther. 29, 324 (2021) [PMC7791009]
- Cisplatin resistance of NSCLC cells involves upregulation of visfatin through activation of its transcription and stabilization of mRNA: Z. Lu, et al.; Chem. Biol. Interact, 351, 109705 (2022) [PMID: 34656559]
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