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AdipoGen Life Sciences
Nampt (Visfatin/PBEF) (human) (IntraCellular) ELISA Kit
660
CHF
CHF 660.00
In stock
AG-45A-0008YEK-KI0196 wellsCHF 660.00
Figure 1: Standard curve
| Product Details | |
|---|---|
| Synonyms | Nicotinamide Phosphoribosyltransferase; PBEF1; Pre-B Cell Colony Enhancing Factor 1; Intracellular Nampt |
| Product Type | Kit |
| Properties | |
| Application Set | Quantitative ELISA |
| Specificity | Detects human Nampt. Weakly cross-reacts (<15%) with mouse Nampt and rat Nampt. Does not cross-react with human adiponectin, human resistin, human RELM-β or human leptin. |
| Crossreactivity | Human |
| Quantity | 1 x 96 wells |
| Sensitivity | 30pg/ml |
| Range | 0.25 to 16ng/ml |
| Sample Type | Cell Lysate |
| Assay Type | Sandwich |
| Detection Type | Colorimetric |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | +4°C |
| Handling Advice |
After standard reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Plate and reagents should reach room temperature before use. |
| Use/Stability | 12 months after the day of manufacturing. See expiry date on ELISA Kit box. |
| Documents | |
| Manual |
 Download PDF |
| MSDS |
Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Nicotinamide phosphoribosyltransferase (Nampt; pre-B cell colony-enhancing factor; PBEF; Visfatin) is an 52kDA adipokine secreted by adipose tissue and involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). Two forms of Nampt exist, an intracellular form (iNampt) and an extracellular form (eNampt). While the function of iNampt as an essential and rate-limiting NAD+ biosynthetic enzyme is well established, the physiological role of eNampt is still a matter of debate. It has been shown that Nampt expression is increased in different diseases, like diabetes, obesity, fetal growth retardation, sepsis, inflammatory bowel disease, Crohn’s disease and rheumatoid arthritis. Furthermore, Nampt might be a marker of pre-eclampsia and of endothelial health.
Product References
- Nicotinamide phosphoribosyltransferase (NAMPT/PBEF/visfatin) is constitutively released from human hepatocytes: A. Garten, et al.; BBRC 391, 376 (2010)
- Leucocytes are a major source of circulating nicotinamide phosphoribosyltransferase (NAMPT)/pre-B cell colony (PBEF)/visfatin linking obesity and inflammation in humans: D. Friebe, et al.; Diabetologia 54, 1200 (2011)
- Visceral adipose tissue visfatin in nonalcoholic fatty liver disease: R. Gaddipati, et al.; Ann. Hepatol. 9, 266 (2010)
- In vivo Suppression of Visfatin by Oral Glucose Uptake: Evidence for a Novel Incretin-Like Effect by Glucagon-Like Peptide-1 (GLP-1): M. Bala, et al.; J. Clin. Endocrinol. Metab. 96, 2493 (2011)
- Cisplatin resistance of NSCLC cells involves upregulation of visfatin through activation of its transcription and stabilization of mRNA: Z. Lu, et al.; Chem. Biol. Interact, 351, 109705 (2021)
- CTRP13 Mitigates Abdominal Aortic Aneurysm Formation via NAMPT1: W. Xu, et al.; Mol. Ther. 29, 324 (2021)
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