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AdipoGen Life Sciences
Nampt (Visfatin/PBEF) (human) ELISA Kit
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650
CHF
CHF 650.00
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AG-45A-0006YEK-KI0196 wellsCHF 650.00
AG-45A-0006YTP-KI012 x 96 wellsCHF 1’170.00
Figure 1: Standard curve
Specifications / Handling
| Product Details | |
|---|---|
| Synonyms | Nicotinamide Phosphoribosyltransferase; PBEF1; Pre-B Cell Colony Enhancing Factor 1 |
| Product Type | Kit |
| Properties | |
| Application Set | Quantitative ELISA |
| Specificity |
Detects human Nampt. Weakly cross-reacts (<15%) with mouse Nampt and rat Nampt. Does not cross-react with human adiponectin, human resistin, human vaspin or human RBP4. Has been shown to specifically detect the physiologically relevant Nampt dimer in human serum samples. |
| Crossreactivity | Human |
| Quantity |
1 x 96 wells |
| Sensitivity | 30pg/ml |
| Range | 0.125 to 8ng/ml |
| Sample Type |
Plasma Serum |
| Assay Type | Sandwich |
| Detection Type | Colorimetric |
| Other Product Data |
Note: Has been optimized to work also in plasma samples. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | +4°C |
| Handling Advice |
After standard reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Plate and reagents should reach room temperature before use. |
| Use/Stability | 12 months after the day of manufacturing. See expiry date on ELISA Kit box. |
| Documents | |
| Manual |
 Download PDF |
| MSDS |
Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Scientific Background Information
Product Description
Nicotinamide phosphoribosyltransferase (NAMPT; Visfatin; PBEF) is a highly conserved protein with important functions in cellular metabolism, inflammation and disease. Intracellular NAMPT (iNAMPT) is the rate-limiting enzyme of the NAD+ salvage pathway and is essential for maintaining cellular NAD+ levels and the activity of NAD+-dependent enzymes. In addition, NAMPT is actively released into the extracellular space. Extracellular NAMPT (eNAMPT) acts as an adipocytokine and immunometabolic signaling molecule and has been linked to pro-inflammatory, pro-angiogenic and tissue-remodeling processes.
Circulating eNAMPT levels are altered in a broad range of metabolic and inflammatory disorders, making NAMPT an increasingly studied biomarker at the interface of metabolism and immunity. Elevated circulating NAMPT has been associated with obesity, insulin resistance and type 2 diabetes, metabolic syndrome and fatty liver disease, as well as cardiovascular and vascular inflammatory conditions including atherosclerosis. NAMPT is also implicated in chronic inflammatory diseases such as inflammatory bowel disease and rheumatoid arthritis, and increased circulating levels have been reported in acute inflammatory conditions including sepsis and lung injury.
NAMPT is additionally emerging as a biomarker and therapeutic target in cancer. Increased NAMPT expression and circulating eNAMPT levels have been associated with several malignancies and with tumor-promoting inflammation, metabolic adaptation, angiogenesis and disease progression. Measurement of circulating NAMPT can therefore be useful for translational studies investigating the relationship between systemic metabolism, inflammation and tumor biology.
The NAMPT (Visfatin/PBEF) (human) ELISA Kit is designed for the quantitative determination of human NAMPT in serum and plasma and specifically detects the physiologically relevant NAMPT dimer.
Typical biomarker research applications include:
• Obesity, metabolic syndrome, insulin resistance and type 2 diabetes
• Fatty liver and metabolic liver disease
• Cardiovascular disease, endothelial dysfunction and atherosclerosis
• Acute and chronic inflammatory diseases
• Sepsis and inflammatory lung injury
• Cancer metabolism, tumor-associated inflammation and cancer progression
• Pregnancy-associated metabolic and vascular disorders such as pre-eclampsia
• Monitoring changes in circulating NAMPT during metabolic, nutritional or therapeutic interventions
Product-specific References
- Visfatin is present in bovine mammary epithelial cells, lactating mammary gland and milk, and its expression is regulated by cAMP pathway: T. Yonezawa, et al.; FEBS Lett. 580, 6635 (2006) [PMID: 17123517]
- Molecular characteristics of serum visfatin and differential detection by immunoassays: A. Koerner, et al.; J. Clin. Endocrinol. Metab. 92, 4783 (2007) [PMID: 17878256]
- Serum levels of the adipokine visfatin are increased in pre-eclampsia: M. Fasshauer, et al.; Clin. Endocrinol. 69, 69 (2008) [PMID: 18034779]
- Correlation of circulating full-length visfatin (PBEF/Nampt) with metabolic parameters in subjects with and without diabetes: a cross-sectional study: R. Retnakaran, et al.; Clin. Endocrinol. 69, 885 (2008) [PMID: 18410550]
- Longitudinal changes in pancreatic and adipocyte hormones following Roux-en-Y gastric bypass surgery: M.M. Swarbrick, et al.; Diabetologia 51, 1901 (2008) [PMC4601635]
- Adipokines influencing metabolic and cardiovascular disease are differentially regulated in maintenance hemodialysis: M. Ziegelmeier, et al.; Metabolism 57, 1414 (2008) [PMID: 18803947]
- Serum visfatin and its relation to insulin resistance and inflammation in type 2 diabetic patients with and without macroangiopathy: A.A. Alghasham & Y.A. Barakat; Saudi Med. J. 29, 185 (2008) [PMID: 18246224]
- Ethnic-specific Correlations of Visfatin With Circulating Markers of Endothelial Inflammation and Function: M. Reimann, et al.; Obesity 17, 2210 (2009) [PMID: 19444230]
- Visfatin activates eNOS via Akt and MAP kinases and improves endothelial cell function and angiogenesis in vitro and in vivo: translational implications for atherosclerosis: F. Lovren, et al.; Am. J. Physiol. Endocrinol. Metab. 296, E1440 (2009) [PMID: 19351806]
- Circulating visfatin in chronic obstructive pulmonary disease: X. Liu, et al.; Nutrition 25, 373 (2009) [PMID: 19056239]
- Visfatin in gestational diabetes: serum level and mRNA expression in fat and placental tissue: B. Telejko, et al.; Diabetes Res. Clin. Pract. 84, 68 (2009) [PMID: 19185944]
- Plasma visfatin concentrations after a lifestyle intervention were directly associated with inflammatory markers: S. Bo, et al.; Nutr. Metab. Cardiovasc. Dis. 19, 423 (2009) [PMID: 19073361]
- Plasma visfatin and ghrelin response to prolonged sculling in competitive male rowers: J. Jurimae, et al.; Med. Sci. Sports Exerc. 41, 137 (2009) [PMID: 19092696]
- Adipokines and systemic lupus erythematosus: relationship with metabolic syndrome and cardiovascular disease risk factors: M. Vadacca, et al.; J. Rheumatol. 36, 295 (2009) [PMID: 19132788]
- Active visfatin is elevated in serum of maintenance haemodialysis patients and correlates inversely with circulating HDL cholesterol: K.D. Nusken, et al.; Nephrol. Dial. Transplant. 24, 2832 (2009) [PMID: 19389865]
- Alteration of adipokines during peripheral blood stem cell mobilization induced by granulocyte colony-stimulating factor: Y. Tanaka, et al.; J. Clin. Apheresis 24, 205 (2009) [PMID: 19816958]
- Peritoneal fluid adipokines: ready for prime time?: R. Wiest, et al.; Eur. J. Clin. Invest. 39, 219 (2009) [PMID: 19260952]
- Adipocytokine levels in gastric cancer patients: resistin and visfatin as biomarkers of gastric cancer: T.E. Nakajima, et al.; J. Gastroenterol. 44, 685 (2009) [PMID: 19430715]
- Release of 12 Adipokines by Adipose Tissue, Nonfat Cells, and Fat Cells From Obese Women: J.N. Fain, et al.; Obesity 18, 890 (2010) [PMID: 19834460]
- Visfatin is a positive regulator of MCP-1 in human adipocytes in vitro and in mice in vivo: G. Sommer, et al.; Obesity 18, 1486 (2010) [PMID: 20035281]
- A 12-week regimen of caloric restriction improves levels of adipokines and pro-inflammatory cytokines in Korean women with BMIs greater than 23 kg/m2: I.S. Lee, et al.; Inflamm. Res. 59, 399 (2010) [PMID: 19916070]
- Nicotinamide phosphoribosyltransferase (NAMPT/PBEF/visfatin) is constitutively released from human hepatocytes: A. Garten, et al.; BBRC 391, 376 (2010) [PMID: 19912992]
- The relationship between visfatin and metabolic syndrome in postmenopausal women: J.H. Kim, et al.; Maturitas 67, 67 (2010) [PMID: 20537826]
- Transcriptome analysis of monocyte-HIV interactions: R. Van den Bergh, et al.; Retrovirology 7, 53 (2010) [PMC2900222]
- Adipokines and cardiometabolic function: How are they interlinked?: A.E. Schutte, et al.; Regul. Pept. 164, 133 (2010) [PMID: 20615436]
- Splanchnic concentrations and postprandial release of visceral adipokines: R. Wiest, et al.; Metabolism 59, 664 (2010) [PMID: 19913846]
- Adipocytokines as new promising markers of colorectal tumors: adiponectin for colorectal adenoma, and resistin and visfatin for colorectal cancer: T.E. Nakajima, et al.; Cancer Sci. 101, 1286 (2010) [PMID: 20331631]
- Adipocytokines and bone mineral density in adolescent female athletes: R. Gruodyte, et al.; Acta Paediatr. 99, 1879 (2010) [PMID: 20545933]
- Serum visfatin is associated with type 2 diabetes mellitus independent of insulin resistance and obesity: A. Esteghamati, et al.; Diabetes Res. Clin. Pract. 91, 154 (2011) [PMID: 21122936]
- Plasma visfatin and adiponectin concentrations in physically active adolescent girls: relationships with insulin sensitivity and body composition variables: J. Jurimae, et al.; JPEM 24, 419 (2011) [PMID: 21932575]
- Serum visfatin and vaspin levels in prepubertal children: effect of obesity and weight loss after behavior modifications on their secretion and relationship with glucose metabolism: G.A. Martos-Moreno, et al.; Int. J. Obes. (Lond) 35, 1355 (2011) [PMID: 21266955]
- Admission Visfatin Levels Predict Pancreatic and Peripancreatic Necrosis in Acute Pancreatitis and Correlate With Clinical Severity: A. Schaeffler, et al.; Am. J. Gastroenterol. 106, 957 (2011) [PMID: 21245835]
- Circulating Nampt and RBP4 levels in patients with carotid stenosis undergoing carotid endarterectomy (CEA): G. Aust, et al.; Clin. Chim. Acta 412, 1195 (2011) [PMID: 21406189]
- The effect of omega-3 on the serum visfatin concentration in patients with type II diabetes: H. Hajianfar, et al.; JRMS 16, 490 (2011) [PMC3214353]
- Pre-B-cell colony-enhancing factor and its clinical correlates with acute lung injury and sepsis: K.A. Lee & M.N. Gong; Chest 140, 382 (2011) [Plasma Samples] [PMC3148796]
- Full-length visfatin levels are associated with inflammation in women with polycystic ovary syndrome: T.K. Lajunen, et al.; Eur. J. Clin. Invest. 42, 321 (2012) [PMID: 21913913]
- Possible role of visfatin in hepatoma progression and the effects of branched-chain amino acids on visfatin-induced proliferation in human hepatoma cells: S. Ninomiya, et al.; Cancer Prev. Res. (Phila) 4, 2092 (2011) [PMID: 21952585]
- Type 2 diabetes and lipoprotein metabolism affect LPS-induced cytokine and chemokine release in primary human monocytes: M. Bala, et al.; Exp. Clin. Endocrinol. Diabetes 119, 370 (2011) [PMID: 21104588]
- Compare the effects of different visfatin concentration on cardiovascular risk factors, adiponectin and insulin resistance in patients with T2DM: S. Mohammadi, et al.; Diab. Metab. Syndr. 5, 71 (2011) [PMID: 22813406]
- Visfatin is secreted into the breast milk and is correlated with weight changes of the infant after the birth: J. Bienertova-Vasku, et al.; Diabetes Res. Clin. Pract. 96, 355 (2012) [PMID: 21741723]
- Increased levels and adipose tissue expression of visfatin in morbidly obese women. The relationship with pro-inflammatory cytokines: X. Terra, et al.; Clin. Endocrinol. (Oxf.) 77, 691 (2012) [PMID: 22182404]
- Serum visfatin concentrations in gestational diabetes mellitus and normal pregnancy: N. Rezvan, et al.; Arch. Gynecol. Obstet. 285, 1257 (2012) [PMID: 22167446]
- Serum visfatin and vaspin levels in normoglycemic first-degree relatives of Iranian patients with type 2 diabetes mellitus: S. Akbarzadeh, et al.; Diabetes Res. Clin. Pract. 95, 132 (2012) [PMID: 22024288]
- Extracellular ATP induces P2X7-dependent nicotinamide phosphoribosyltransferase release in LPS-activated human monocytes: E. Schilling & S. Hauschildt; Innate Immun. 18, 738 (2012) [PMID: 22377803]
- The Value of Visfatin in the Prediction of Metabolic Syndrome: A Multi-Factorial Analysis: A. Esteghamati, et al.; J. Cardiovasc. Transl. Res. 5, 541 (2012) [PMID: 22585407]
- The effects of omega-3 on blood pressure and the relationship between serum visfatin level and blood pressure in patients with type II diabetes: M.J. Hosseinzadeh-Attar, et al.; ARYA Atheroscler. 8, 1 (2012) [PMC3448398]
- Association of sleep onset of acute coronary syndrome with sleep apnea syndrome and abnormal diurnal variation of hemostasis and adipokine levels: Y. Yano, et al.; Blood Coagul. Fibrinolysis 23, 590 (2012) [PMID: 22828597]
- Omentin-1, visfatin and adiponectin levels in relation to bone mineral density in Iranian postmenopausal women: M. Tohidi, et al.; Bone 51, 876 (2012) [PMID: 22971441]
- Lipid Profiles and Serum Visfatin Concentrations in Patients with Type II Diabetes in Comparison with Healthy Controls: H. Hajianfar, et al.; Int. J. Prev. Med. 3, 326 (2012) [PMC3372075]
- Effect of substituting saturated with monounsaturated fatty acids on serum visfatin levels and insulin resistance in overweight women: A randomized cross-over clinical trial: F. Haghighatdoost, et al.; Int. J. Food Sci. Nutr. 63, 772 (2012) [PMID: 22375890]
- Plasma visfatin levels and gene expression in morbidly obese women with associated fatty liver disease: T. Auguet, et al.; Clin. Biochem. 46, 202 (2013) [PMID: 23174488]
- Portal and systemic levels of visfatin in morbidly obese subjects undergoing bariatric surgery: Z. Karbaschian, et al.; Endocrine 44, 114 (2013) [PMID: 23104149]
- Associations of metabolic factors and adipokines with pain in incipient upper extremity soft tissue disorders: a cross-sectional study: M. Rechardt, et al.; BMJ Open 3, e003036 (2013) [PMC3753506]
- Admission plasma visfatin level strongly correlates with hematoma growth and early neurologic deterioration in patients with acute spontaneous basal ganglia hemorrhage: S.J. Gu, et al.; Clin. Chim. Acta 425, 85 (2013) [PMID: 23916712]
- Effect of weight reduction following bariatric surgery on serum visfatin and adiponectin levels in morbidly obese subjects: M.J. Hosseinzadeh-Attar, et al.; Obes. Facts 6, 193 (2013) [PMC5644781]
- Expression and regulation of nampt in human islets: K. Kover, et al.; PLoS One 8, e58767 (2013) [PMC3594147]
- Visfatin as a Novel Mediator Released by Inflamed Human Endothelial Cells: T. Romacho, et al.; PLoS One 8, e78283 (2013) [PMC3795064]
- Adipocytokine profile, cytokine levels and foxp3 expression in multiple sclerosis: a possible link to susceptibility and clinical course of disease: S. Emamgholipour, et al.; PLoS One 8, e76555 (2013) [PMC3789814]
- Associations between tissue visfatin/nicotinamide phosphoribosyltransferase (Nampt), retinol binding protein-4, and vaspin concentrations and insulin resistance in morbidly obese subjects: Z. Goktas, et al.; Mediators Inflamm. 2013, 861496 (2013) [PMC3866788]
- Expression and function of visfatin (Nampt), an adipokine-enzyme involved in inflammatory pathways of osteoarthritis: M.C. Laiguillon, et al.; Arthritis Res. Ther. 16, R38 (2014) [PMC3978827]
- Identification of adipokine clusters related to parameters of fat mass, insulin sensitivity and inflammation: G. Flehmig, et al.; PLoS One 9, e99785 (2014) [PMC4072672]
- Extracellular nicotinamide phosphoribosyltransferase (NAMPT) promotes M2 macrophage polarization in chronic lymphocytic leukemia: V. Audrito, et al.; Blood 125, 111 (2015) [Plasma Samples] [PMID: 25368373]
- Ethnic differences in the role of adipocytokines linking abdominal adiposity and insulin sensitivity among Asians: E. Parvaresh Rizi, et al.; J. Clin. Endocrinol. Metab. 100, 4249 (2015) [Plasma Samples] [PMID: 26308293]
- Nampt/PBEF/visfatin serum levels: a new biomarker for retinal blood vessel occlusions: S. Kaja, et al.; Clin. Ophthalmol. 9, 611 (2015) [PMC4396426]
- Adipo/cytokines in atherosclerotic secretomes: increased visfatin levels in unstable carotid plaque: T. Auguet, et al.; BMC Cardiovasc. Disord. 16, 149 (2016) [PMC4939016]
- Nicotinamide phosphoribosyltransferase production in human spermatozoa is influenced by maturation stage: S. Riammer, et al.; Andrology 4, 1045 (2016) [Semen Samples] [PMID: 27566659]
- Prediction of Nonalcoholic Fatty Liver Disease Via a Novel Panel of Serum Adipokines: R. Jamali, et al.; Medicine (Baltimore) 95, e2630 (2016) [PMC4748893]
- Serum adipokines might predict liver histology findings in non-alcoholic fatty liver disease: R. Jamali, et al.; World J. Gastroenterol. 22, 5096 (2016) [PMC4886385]
- Nicotinamide Phosphoribosyltransferase Promotes Pulmonary Vascular Remodeling and Is a Therapeutic Target in Pulmonary Arterial Hypertension: J. Chen, et al.; Circulation 135, 1532 (2017) [PMC5400780]
- Anti-Inflammatory and Pro-Inflammatory Adipokine Profiles in Children on Vegetarian and Omnivorous Diets: J. Ambroszkiewicz, et al.; Nutrients 10, 1241 (2018) [PMC6164316]
- Umbilical Cord Blood and Maternal Visfatin (PBEF/Nampt) Concentrations in Preterm Birth with and without Preterm Premature Rupture of Membranes: T. Pavlova, et al.; J. Matern. Fetal Neonatal Med. 31, 1811 (2018) [PMID: 28482725]
- Circulating adipokines and mRNA expression in adipose tissue and the placenta in women with gestational diabetes mellitus: P.C. Tsiotra, et al.; Peptides 101, 157 (2018) [PMID: 29337272]
- Extracellular nicotinamide phosphoribosyltransferase (eNAMPT) is a novel marker for patients with BRAF-mutated metastatic melanoma: V. Audrito, et al.; Oncotarget 9, 18997 (2018) [Plasma & Cell Culture Supernatant Samples] [PMC5922372]
- Extracellular nicotinate phosphoribosyltransferase binds Toll like receptor 4 and mediates inflammation: A. Managò, et al.; Nat. Commun. 10, 4116 (2019) [PMID: 31511522]
- Neutralization of extracellular NAMPT (nicotinamide phosphoribosyltransferase) ameliorates experimental murine colitis: G. Colombo, et al.; J. Mol. Med. 98, 595 (2020) [PMID: 32338310]
- Nicotinamide phosphoribosyltransferase as a biomarker for the diagnosis of infectious pleural effusions: J. Huang, et al.; Sci. Rep. 11, 21121 (2021) [PMC8548599]
- Adiponectin Associates with Rheumatoid Arthritis Risk in Overweight and Obesity Independently of Other Adipokines: Y. Zhang, et al.; J. Clin. Med. 10, 2791 (2021) [PMC8267689]
- Extracellular nicotinamide phosphoribosyltransferase boosts IFNγ-induced macrophage polarization independently of TLR4: G. Colombo, et al.; iScience 25, 104147 (2022) [PMC8990213]
- Relationships between Body Weight Status and Serum Levels of Adipokine, Myokine and Bone Metabolism Parameters in Healthy Normal Weight and Thin Children: J. Ambroszkiewicz, et al.; J. Clin. Med. 11, 4013 (2022) [PMC9320693]
- Osteo-immunological impact of radon spa treatment: due to radon or spa alone? Results from the prospective, thermal bath placebo-controlled RAD-ON02 trial: D. Eckert, et al.; Front. Immunol. 14, 1284609 (2023) [PMC10824901]
- Impact of holder pasteurization on protein and eNAMPT/Visfatin content in human breast milk: A. Givonetti, et al.; Sci. Rep. 14, 29246 (2024) [PMC11589111]
- Inhibition of ethanol-induced eNAMPT secretion attenuates liver ferroptosis through BAT-Liver communication: Y. Zhou, et al.; Redox Biol. 75, 103274 (2024) [PMC11327441]
- Leptin: a gender and obesity-related marker predictive of metabolic comorbidities and therapeutic response to anti-IL-23 biologic drugs in psoriatic patients: R. Belli, et al.; Front. Immunol. 16, 1607312 (2025) [PMC12307160]
- Can Circulating MicroRNAs, Cytokines, and Adipokines Help to Differentiate Psoriatic Arthritis from Erosive Osteoarthritis of the Hand? A Case-Control Study: A. Fioravanti, et al.; Int. J. Mol. Sci. 26, 4621 (2025) [PMC12111288]
- Anti-IL-17 and Anti-IL-23 Therapies Modulate Serum Biomarkers of Intestinal Dysbiosis and Oxidative Stress Linked to Cardiovascular Risk in Patients with Psoriasis: G. Annunziata, et al.; Life (Basel) 15, 1703 (2025) [PMC12653977]
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![Measurement of NAMPT enzymatic activity was performed as described previously [G.C. Elliott, et al.; Anal Biochem 107, 199 (1980)]. The recombinant Nampt was diluted in assay buffer and 10μl per 50μl reaction mix were applied in the reaction mix (20](https://adipogen.com/media/catalog/product/cache/60eb5af712bc93baae8d55513bd31b01/a/g/ag-40a-0018_h-nampt_visfatin_-his-enzymatic-activity.png)