AdipoGen Life Sciences

Nampt (Visfatin/PBEF) (human) (rec.) (His) (BULK)

CHF 2'800.00
In stock
AG-40A-0018AA-C500500 µgCHF 2'800.00
More Information
Product Details
Synonyms Pre-B Cell Colony Enhancing Factor 1; PBEF1; Nicotinamide Phosphoribosyltransferase
Product Type Protein
Properties
Source/Host E. coli
Sequence

Intracellular human Nampt (visfatin/PBEF) (aa 1-491) is fused at the C-terminus to a His-tag.

Crossreactivity Human
Biological Activity

Shows adipogenic effects in stimulated differentiating 3T3-L1 cells.

MW ~52kDa (SDS-PAGE)
Purity ≥90% (SDS-PAGE)
Endotoxin Content <1EU/μg purified protein (LAL test; Lonza).
Reconstitution Reconstitute in PBS.
Formulation Lyophilized.
Other Product Data

UniProt link P43490: Nicotinamide phosphoribosyltransferase (human)

Shipping and Handling
Shipping BLUE ICE
Short Term Storage -20°C
Long Term Storage -20°C
Handling Advice After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

Nicotinamide phosphoribosyltransferase (Nampt; pre-B cell colony-enhancing factor; PBEF; Visfatin) is an 52kDa adipokine secreted by adipose tissue and involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). Two forms of Nampt exist, an intracellular form (iNampt) and an extracellular form (eNampt). While the function of iNampt as an essential and rate-limiting NAD+ biosynthetic enzyme is well established, the physiological role of eNampt is still a matter of debate. Nampt has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity. Recently NAMPT extracellular (eNAMPT) has been shown to be secreted from macrophages in muscles and to triggers a proliferative signal of muscle stem cell (MusC) by binding to CCR5 located at the surface receptor of MusC.

Product References
  1. Visfatin is present in bovine mammary epithelial cells, lactating mammary gland and milk, and its expression is regulated by cAMP pathway: T. Yonezawa, et al.; FEBS Lett. 580, 6635 (2006) 
  2. Molecular characteristics of serum visfatin and differential detection by immunoassays: A. Körner, et al.; J. Clin. Endocrinol. Metab. 92, 4783 (2007) Lack of direct insulin-like action of visfatin/Nampt/PBEF1 in human adipocytes: E. Wanecq, et al.; J. Physiol. Biochem. 65, 351 (2009)
  3. Nicotinamide phosphoribosyltransferase (NAMPT/PBEF/visfatin) is a tumoural cytokine released from melanoma: A.A. Grolla, et al.; Pigment Cell Melanoma Res. 28, 718 (2015)
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