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AdipoGen Life Sciences
Nampt (Visfatin/PBEF) (human) (rec.) (His) (BULK)
As low as
2800
CHF
CHF 2’800.00
In stock
AG-40A-0018AA-C500500 µgCHF 2’800.00

Figure 1: Dimer formation of recombinant Nampt (Visfatin, PBEF) (AG-40A-0018). Purified Nampt (Visfatin, PBEF) was separated by SDS-PAGE and Western blot analysis was performed using rabbit anti-Nampt polyclonal antibody (AG-25A-0025). In the absence of DTT, Nampt (visfatin, PBEF) formed a homodimer, a homomultimer.
Figure 2: Insulin-mimetic effects on stimulated differentiating 3T3-L1 cells.
10μg/ml iNampt (human) (rec.) (His) (Prod. No. AG-40A-0018AA) or human insulin was added to differentiating 3T3-L1 cells that had been stimulated with 1μM dexamethasone and 0.5mM IBMX for 2 days. After 5 days, fat droplets were stained with Oil-Red O.
10μg/ml iNampt (human) (rec.) (His) (Prod. No. AG-40A-0018AA) or human insulin was added to differentiating 3T3-L1 cells that had been stimulated with 1μM dexamethasone and 0.5mM IBMX for 2 days. After 5 days, fat droplets were stained with Oil-Red O.
Figure 3: Measurement of NAMPT enzymatic activity was performed as described previously [G.C. Elliott, et al.; Anal Biochem 107, 199 (1980)]. The recombinant Nampt was diluted in assay buffer and 10μl per 50μl reaction mix were applied in the reaction mix (20 mmol/l TrisHCl pH 7.4; 2,5 mmol/l ATP; 50 mmol/l NaCl; 12,5 mmol/l MgCl2; 2 mmol/l DTT; 0,5 mmol/l PRPP; 5 μmol/l 14C-nicotinamide) and incubated at 37°C for 1h. The 50μl reaction mix was transferred into tubes containing 2ml of acetone and afterwards pipetted onto acetone-presoaked glass microfibre filters (GF/A Ø 24 mm). After rinsing with 2 x 1ml acetone, filters were dried, transferred into vials with 6ml scintillation cocktail and radioactivity of 14C-NMN was quantified in a liquid scintillation counter. After subtraction of buffer values as background, cpm were normalized to 106 cells and the volume of enzyme preparation (10μl). Mouse liver lysate at a concentration of 34.5μg/ml was used as positive control in each assay. The positive control is mouse liver lysate at a concentration of 34,5μg/ml – normally, which brings the most counts per minute (cpm) (contributed by Antje Garten & Dr. Kiess, University of Leipzig, Germany).
Product Details | |
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Synonyms | Pre-B Cell Colony Enhancing Factor 1; PBEF1; Nicotinamide Phosphoribosyltransferase |
Product Type | Protein |
Properties | |
Source/Host | E. coli |
Sequence |
Intracellular human Nampt (visfatin/PBEF) (aa 1-491) is fused at the C-terminus to a His-tag. |
Crossreactivity | Human |
Biological Activity |
Shows adipogenic effects in stimulated differentiating 3T3-L1 cells. |
MW | ~52kDa (SDS-PAGE) |
Purity | ≥90% (SDS-PAGE) |
Endotoxin Content | <1EU/μg purified protein (LAL test; Lonza). |
Reconstitution | Reconstitute in PBS. |
Formulation | Lyophilized. |
Other Product Data |
UniProt link P43490: Nicotinamide phosphoribosyltransferase (human) |
Shipping and Handling | |
Shipping | BLUE ICE |
Short Term Storage | -20°C |
Long Term Storage | -20°C |
Handling Advice |
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. |
Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
Documents | |
MSDS |
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Product Specification Sheet | |
Datasheet |
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