AdipoGen Life Sciences

anti-Nampt (Visfatin/PBEF), mAb (OMNI379) (Biotin)

CHF 350.00

In stock

AG-20A-0034B-C05050 µgCHF 350.00

Figure 1: Immunohistochemical staining of Nampt using anti-Nampt (Visfatin/PBEF), mAb (OMNI379) (Prod. No. AG-20A-0034B) in formalin-fixed and paraffin-embedded (FFPE) human colon tissue (15µg/ml).

Figure 2: Immunohistochemical staining of Nampt using anti-Nampt (OMNI379) (Prod. No. AG-20A-0034B) in human kidney tissue (1:500 dilution). Immunoperoxidase staining of formalin-fixed and paraffin-embedded (FFPE) human kidney showing cytoplasmic staining (200x, brown color).

Figure 3: Immunofluorescent confocal imaging of HEK293 cells using anti-Nampt (Visfatin-PBEF), mAb (OMNI379) (Prod. No. AG-20A-0034B). Nampt (Visfatin/PBEF) (green staining) is localized in the cytoplasm and nucleus (400x magnification). Nuclei are stained with Hoechst (blue staining).

Figure 4: Western blot analysis using anti-Nampt (Visfatin-PBEF), mAb (OMNI379) (Prod. No. AG-20A-0034B) at 1:2000 dilution.
1. Human Nampt (Visfatin/PBEF) (His-tagged).
2. Human Nampt (Visfatin/PBEF) (FLAG^®-tagged).
3. Mouse Nampt (Visfatin/PBEF) (His-tagged).
4. Rat Nampt (Visfatin/PBEF) (His-tagged).
5. Human Nampt (Visfatin/PBEF) (His-tagged).
6. LPS-treated human peripheral blood leukocyte lysate.

Figure 5: Western blot analysis of 3T3-L1 cell lysate using anti-Nampt (Visfatin/PBEF) mAb (OMNI379) (Prod. No. AG-20A-0034B) at 1:2000 dilution.
1. IBMX. + DEX. - treated 3T3-L1 cell lysate.
2. IBMX. + DEX. + Insulin - treated 3T3-L1 cell lysate.

Specifications / Handling

More Information
Product Details
Synonyms	Pre-B Cell Colony Enhancing Factor 1; PBEF1; Nicotinamide Phosphoribosyltransferase
Product Type	Monoclonal Antibody
Properties
Clone	OMNI379
Isotype	Mouse IgG2aκ
Immunogen/Antigen	Recombinant human Nampt (visfatin/PBEF).
Label/Conjugates	Biotin
Application	ELISA: (direct or indirect: 1:2'000-1:10'000) Immunocytochemistry: (1:500-1:1’000) Immunohistochemistry: (paraffin sections, 1:100-1:500) Western blot: (1:2'000-1:5'000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature). Optimal conditions should be determined individually for each application.
Crossreactivity	Human Mouse Rat
Specificity	Recognizes human, mouse and rat Nampt.
Purity Detail	Protein G-affinity purified.
Concentration	1mg/ml
Formulation	Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Isotype Negative Control	Mouse IgG2a Isotype Control (Biotin)
RRID	AB_2490118
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Nicotinamide phosphoribosyltransferase (Nampt; pre-B cell colony-enhancing factor; PBEF; Visfatin) is an 52kDa adipokine secreted by adipose tissue and involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). Two forms of Nampt exist, an intracellular form (iNampt) and an extracellular form (eNampt). While the function of iNampt as an essential and rate-limiting NAD+ biosynthetic enzyme is well established, the physiological role of eNampt is still a matter of debate. Nampt has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.

Product References

Hypoxic induction of human visfatin gene is directly mediated by hypoxia-inducible factor-1: S.K. Bae, et al.; FEBS Lett. 580, 4105 (2006)
Molecular Characteristics of Serum Visfatin and Differential Detection by Immunoassays: A. Korner, et al.; J. Clin. Endocrinol. Metab. 92, 4783 (2007)
Nampt/PBEF/Visfatin Regulates Insulin Secretion in beta Cells as a Systemic NAD Biosynthetic Enzyme: J.R. Revollo, et al.; Cell. Metab. 6, 363 (2007)
Nicotinamide phosphoribosyltransferase (NAMPT/PBEF/visfatin) is constitutively released from human hepatocytes: A. Garten, et al.; BBRC 391, 376 (2010)
Intracellular nicotinamide phosphoribosyltransferase protects against hepatocyte apoptosis and is down-regulated in nonalcoholic fatty liver disease: T.B. Dahl, et al.; J. Clin. Endocrinol. Metab. 95, 3039 (2010) Supplement Information
Expression and regulation of nampt in human islets: K. Kover, et al.; PLoS One 8, e58767 (2013)
Extracellular nicotinamide phosphoribosyltransferase (NAMPT) promotes M2 macrophage polarization in chronic lymphocytic leukemia: V. Audrito, et al.; Blood 125, 111 (2015)
SIRT1-Mediated eNAMPT Secretion from Adipose Tissue Regulates Hypothalamic NAD+ and Function in Mice: M.J. Yoon, et al.; Cell Metab. 21, 706 (2015)
Nicotinamide phosphoribosyltransferase production in human spermatozoa is influenced by maturation stage: S. Riammer, et al.; Andrology 4, 1045 (2016)
Synthesis and degradation of adenosine 50-tetraphosphate by nicotinamide and nicotinate phosphoribosyltransferases: A. Amici, et al.; Cell Chem. Biol. 24, 553 (2017)
Nicotinamide phosphoribosyltransferase expression and clinical outcome of resected stage I/II pancreatic ductal adenocarcinoma: K. Davis, et al.; PLoS One 14, e0213576 (2019)
A nicotinamide phosphoribosyltransferase-GAPDH interaction sustains the stress-induced NMN/NAD+ salvage pathway in the nucleus: A.A. Grolla, et al.; J. Biol. Chem. 295, 3635 (2020)

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