AdipoGen Life Sciences

anti-Nampt (Visfatin/PBEF), mAb (OMNI379) (Biotin)

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AG-20A-0034B-C05050 µgCHF 350.00

Figure 1: Immunohistochemical staining of Nampt using anti-Nampt (Visfatin/PBEF), mAb (OMNI379) (Prod. No. AG-20A-0034B) in formalin-fixed and paraffin-embedded (FFPE) human colon tissue (15µg/ml).

Figure 2: Immunohistochemical staining of Nampt using anti-Nampt (OMNI379) (Prod. No. AG-20A-0034B) in human kidney tissue (1:500 dilution). Immunoperoxidase staining of formalin-fixed and paraffin-embedded (FFPE) human kidney showing cytoplasmic staining (200x, brown color).

Figure 3: Immunofluorescent confocal imaging of HEK293 cells using anti-Nampt (Visfatin-PBEF), mAb (OMNI379) (Prod. No. AG-20A-0034B). Nampt (Visfatin/PBEF) (green staining) is localized in the cytoplasm and nucleus (400x magnification). Nuclei are stained with Hoechst (blue staining).

Figure 4: Western blot analysis using anti-Nampt (Visfatin-PBEF), mAb (OMNI379) (Prod. No. AG-20A-0034B) at 1:2000 dilution.
1. Human Nampt (Visfatin/PBEF) (His-tagged).
2. Human Nampt (Visfatin/PBEF) (FLAG^®-tagged).
3. Mouse Nampt (Visfatin/PBEF) (His-tagged).
4. Rat Nampt (Visfatin/PBEF) (His-tagged).
5. Human Nampt (Visfatin/PBEF) (His-tagged).
6. LPS-treated human peripheral blood leukocyte lysate.

Figure 5: Western blot analysis of 3T3-L1 cell lysate using anti-Nampt (Visfatin/PBEF) mAb (OMNI379) (Prod. No. AG-20A-0034B) at 1:2000 dilution.
1. IBMX. + DEX. - treated 3T3-L1 cell lysate.
2. IBMX. + DEX. + Insulin - treated 3T3-L1 cell lysate.

Specifications / Handling

More Information
Product Details
Synonyms	Pre-B Cell Colony Enhancing Factor 1; PBEF1; Nicotinamide Phosphoribosyltransferase
Product Type	Monoclonal Antibody
Properties
Clone	OMNI379
Isotype	Mouse IgG2aκ
Immunogen/Antigen	Recombinant human Nampt (visfatin/PBEF).
Label/Conjugates	Biotin
Application	ELISA: (direct or indirect: 1:2'000-1:10'000) Immunocytochemistry: (1:500-1:1’000) Immunohistochemistry: (paraffin sections, 1:100-1:500) Western blot: (1:2'000-1:5'000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature). Optimal conditions should be determined individually for each application.
Crossreactivity	Human Mouse Rat
Specificity	Recognizes human, mouse and rat Nampt.
Purity Detail	Protein G-affinity purified.
Concentration	1mg/ml
Formulation	Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Isotype Negative Control	Mouse IgG2a Isotype Control (Biotin)
RRID	AB_2490118
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Scientific Background Information

Product Description

Nicotinamide phosphoribosyltransferase (Nampt; pre-B cell colony-enhancing factor; PBEF; Visfatin) is an 52kDa adipokine secreted by adipose tissue and involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). Two forms of Nampt exist, an intracellular form (iNampt) and an extracellular form (eNampt). While the function of iNampt as an essential and rate-limiting NAD+ biosynthetic enzyme is well established, the physiological role of eNampt is still a matter of debate. Nampt has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.

Product-specific References

Hypoxic induction of human visfatin gene is directly mediated by hypoxia-inducible factor-1: S.K. Bae, et al.; FEBS Lett. 580, 4105 (2006) [PMID: 16828081]
Molecular Characteristics of Serum Visfatin and Differential Detection by Immunoassays: A. Korner, et al.; J. Clin. Endocrinol. Metab. 92, 4783 (2007) [PMID: 17878256]
Nampt/PBEF/Visfatin Regulates Insulin Secretion in beta Cells as a Systemic NAD Biosynthetic Enzyme: J.R. Revollo, et al.; Cell. Metab. 6, 363 (2007) [PMC2098698]
Nicotinamide phosphoribosyltransferase (NAMPT/PBEF/visfatin) is constitutively released from human hepatocytes: A. Garten, et al.; BBRC 391, 376 (2010) [PMID: 19912992]
Intracellular nicotinamide phosphoribosyltransferase protects against hepatocyte apoptosis and is down-regulated in nonalcoholic fatty liver disease: T.B. Dahl, et al.; J. Clin. Endocrinol. Metab. 95, 3039 (2010) [PMID: 20392873, Supplement Information]
Expression and regulation of nampt in human islets: K. Kover, et al.; PLoS One 8, e58767 (2013) [PMC3594147]
Extracellular nicotinamide phosphoribosyltransferase (NAMPT) promotes M2 macrophage polarization in chronic lymphocytic leukemia: V. Audrito, et al.; Blood 125, 111 (2015) [PMID: 25368373]
SIRT1-Mediated eNAMPT Secretion from Adipose Tissue Regulates Hypothalamic NAD+ and Function in Mice: M.J. Yoon, et al.; Cell Metab. 21, 706 (2015) [PMC4426056]
Nicotinamide phosphoribosyltransferase production in human spermatozoa is influenced by maturation stage: S. Riammer, et al.; Andrology 4, 1045 (2016) [PMID: 27566659]
Synthesis and degradation of adenosine 5'-tetraphosphate by nicotinamide and nicotinate phosphoribosyltransferases: A. Amici, et al.; Cell Chem. Biol. 24, 553 (2017) [PMID: 28416276]
NAMPT enzyme activity regulates catabolic gene expression in gingival fibroblasts during Periodontitis: K.H. Park, et al.; Exp. Mol. Med. 49, e368 (2017) [PMC5579510]
NAMPT expression in osteoblasts controls osteoclast recruitment in alveolar bone remodeling: B. Hassan, et al.; J. Cell. Physiol. 233, 7402 (2018) [PMC13482124]
Nicotinamide phosphoribosyltransferase expression and clinical outcome of resected stage I/II pancreatic ductal adenocarcinoma: K. Davis, et al.; PLoS One 14, e0213576 (2019) [PMC6411120]
Extracellular Vesicle-Contained eNAMPT Delays Aging and Extends Lifespan in Mice: M. Yoshida, et al.; Cell Metab. 30, 329 (2019) [PMC6687560]
A nicotinamide phosphoribosyltransferase-GAPDH interaction sustains the stress-induced NMN/NAD+ salvage pathway in the nucleus: A.A. Grolla, et al.; J. Biol. Chem. 295, 3635 (2020) [PMC7076215]
Human ESC-sEVs alleviate age-related bone loss by rejuvenating senescent bone marrow-derived mesenchymal stem cells: L. Gong, et al.; J. Extracell. Vesicles 9, 1800971 (2020) [PMC7480439]
Neutralization of extracellular NAMPT (nicotinamide phosphoribosyltransferase) ameliorates experimental murine colitis: G. Colombo, et al.; J. Mol. Med. 98, 595 (2020) [PMID: 32338310]
Tumors carrying BRAF-mutations over-express NAMPT that is genetically amplified and possesses oncogenic properties: V. Audrito, et al.; J. Transl. Med. 20, 118 (2022) [PMC8908704]
Exercise increases the release of NAMPT in extracellular vesicles and alters NAD+ activity in recipient cells: M.C. Chong, et al.; Aging Cell 21, e13647 (2022) [PMC9282849]
Extracellular nicotinamide phosphoribosyltransferase boosts IFNg-induced macrophage polarization independently of TLR4: G. Colombo, et al.; iScience 25, 104147 (2022) [PMC8990213]
Pyruvate transamination and NAD biosynthesis enable proliferation of succinate dehydrogenase-deficient cells by supporting aerobic glycolysis: L. Ricci, et al.; Cell Death Dis. 14, 403 (2023) [PMC10326256]
Identification of structural determinants of nicotinamide phosphoribosyl transferase (NAMPT) activity and substrate selectivity: D. Houry, et al.; J. Struct. Biol. 215, 108004 (2023) [PMID: 37495196]
Nicotinamide phosphoribosyltransferase is a molecular target of potent anticancer agents identified from phenotype-based drug screening: D. Yamaguchi, et al.; Sci. Rep. 9, 7742 (2019) [PMC6533267]
Discovery of small-molecule activators of nicotinamide phosphoribosyltransferase (NAMPT) and their preclinical neuroprotective activity: H. Yao, et al.; Cell Res. 32, 570 (2022) [PMC9160276]
Tumors carrying BRAF-mutations over-express NAMPT that is genetically amplified and possesses oncogenic properties: V. Audrito, et al.; J. Transl. Med. 20, 118 (2022) [PMC8908704]
Hypothalamic astrocyte NAD+ salvage pathway mediates the coupling of dietary fat overconsumption in a mouse model of obesity: J.W. Park, et al.; Nat. Commun. 15, 2102 (2024) [PMC10920699]
An NAMPT Inhibitor Decreases NAMPT Capture by an Antibody Directed against the 5-Phosphoribosyl-1-Pyrophosphate-Binding Loop: A Rational for an NAMPT Occupancy Assay: M. Shomali, et al.; bioRxiv (ePub ahead of Print) (2024) [Preprint]
CD38 is a key mediator of NAD+ depletion in the brain of ZIKV-infected mice: G.N. Saraiva, et al.; iScience 28, 114018 (2025) [PMC12682054]
Extracellular nicotinamide phosphoribosyltransferase (eNAMPT) drives abnormal pericyte-rich vasculature in triple-negative breast cancer: M. Moro, et al.; Angiogenesis 28, 4 (2025) [PMID: 39636369]
Bi-directional regulation between NAD/NAMPT and IFN-γ/PD-L1 axes via BRD4/IRF1 and mitochondrial respiration in metastatic cutaneous melanoma: I. Fiorilla, et al.; J. Exp. Clin. Cancer Res. 45, 147 (2026) [PMC13326467]
Development of PROTACs Targeting the Moonlighting Enzyme Nicotinamide Phosphoribosyltransferase (NAMPT) for Breast Cancer Therapy: U. Galli, et al.; J. Med. Chem. 69, 5259 (2026) [PMC12990041]

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