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AdipoGen Life Sciences
Nampt (Visfatin/PBEF) (mouse) (rec.) (His)
420
CHF
CHF 420.00
In stock
AG-40A-0017-C05050 µgCHF 420.00
Figure 1: Insulin-mimetic effects on stimulated differentiating 3T3-L1 cells.
10μg/ml Nampt (mouse) (rec.) (His) (Prod. No. AG-40A-0017) or human insulin was added to differentiating 3T3-L1 cells that had been stimulated with 1μM dexamethasone and 0.5mM IBMX for 2 days. After 5 days, fat droplets were stained with Oil-Red O.
10μg/ml Nampt (mouse) (rec.) (His) (Prod. No. AG-40A-0017) or human insulin was added to differentiating 3T3-L1 cells that had been stimulated with 1μM dexamethasone and 0.5mM IBMX for 2 days. After 5 days, fat droplets were stained with Oil-Red O.
| Product Details | |
|---|---|
| Synonyms | Pre-B Cell Colony Enhancing Factor 1; PBEF1; Nicotinamide Phosphoribosyltransferase |
| Product Type | Protein |
| Properties | |
| Source/Host | E. coli |
| Sequence | Mouse Nampt (visfatin/PBEF) (aa 1-491) is fused at the C-terminus to a His-tag. |
| Crossreactivity | Mouse |
| Biological Activity | Shows adipogenic effects in stimulated differentiating 3T3-L1 cells. |
| MW | ~52kDa (SDS-PAGE) |
| Purity | ≥90% (SDS-PAGE) |
| Endotoxin Content | <1EU/μg purified protein (LAL test; Lonza). |
| Reconstitution | Reconstitute in distilled water. |
| Formulation | Lyophilized from 0.2μm-filtered solution in PBS, pH 7.2. |
| Other Product Data | UniProt link Q99KQ4: Nicotinamide phosphoribosyltransferase (mouse) |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Nicotinamide phosphoribosyltransferase (Nampt; pre-B cell colony-enhancing factor; PBEF; Visfatin) is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity. Recently NAMPT extracellular (eNAMPT) has been shown to be secreted from macrophages in muscles and to triggers a proliferative signal of muscle stem cell (MusC) by binding to CCR5 located at the surface receptor of MusC.
Product References
- Visfatin regulates insulin secretion, insulin receptor signalling and mRNA expression of diabetes-related genes in mouse pancreatic beta-cells: J.E. Brown, et al.; J. Mol. Endocrinol. 44, 171 (2010)
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