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AdipoGen Life Sciences

anti-Nampt (Visfatin/PBEF), mAb (OMNI379)

CHF 290.00
In stock
AG-20A-0034-C05050 µgCHF 290.00
AG-20A-0034-C100100 µgCHF 410.00
Call  +41 61 926 6040
Fax  +41 61 926 6049
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Figure 1: Immunohistochemical staining of Nampt using anti-Nampt (Visfatin/PBEF), mAb (OMNI379) (Prod. No. AG-20A-0034) in formalin-fixed and paraffin-embedded (FFPE) human colon tissue (15µg/ml).
Figure 2: Immunohistochemical staining of Nampt using anti-Nampt (OMNI379) (Prod. No. AG-20A-0034) in human kidney tissue (1:500 dilution). Immunoperoxidase staining of formalin-fixed and paraffin-embedded (FFPE) human kidney showing cytoplasmic staining (200x, brown color).
Figure 3: Immunofluorescent confocal imaging of HEK293 cells using anti-Nampt (Visfatin-PBEF), mAb (OMNI379) (Prod. No. AG-20A-0034). Nampt (Visfatin/PBEF) (green staining) is localized in the cytoplasm and nucleus (400x magnification). Nuclei are stained with Hoechst (blue staining).
Figure 4: Western blot analysis using anti-Nampt (Visfatin-PBEF), mAb (OMNI379) (Prod. No. AG-20A-0034) at 1:2000 dilution.
1. Human Nampt (Visfatin/PBEF) (His-tagged).
2. Human Nampt (Visfatin/PBEF) (FLAG®-tagged).
3. Mouse Nampt (Visfatin/PBEF) (His-tagged).
4. Rat Nampt (Visfatin/PBEF) (His-tagged).
5. Human Nampt (Visfatin/PBEF) (His-tagged).
6. LPS-treated human peripheral blood leukocyte lysate.
Figure 5: Western blot analysis of 3T3-L1 cell lysate using anti-Nampt (Visfatin/PBEF) mAb (OMNI379) (Prod. No. AG-20A-0034) at 1:2000 dilution.
1. IBMX. + DEX. - treated 3T3-L1 cell lysate.
2. IBMX. + DEX. + Insulin - treated 3T3-L1 cell lysate.
Figure 6: Immunoprecipitation of recombinant human Nampt (Visfatin/PBEF) proteins using anti-Nampt (Visfatin-PBEF), mAb (OMNI379) (Prod. No. AG-20A-0034). Recombinant human Nampt (Visfatin/PBEF) proteins at different concentrations were precipitated. The precipitated proteins were separated by SDS-PAGE, electroblotted and visualized by western blot with anti-human Nampt(Visfatin/PBEF) pAb.
More Information
Product Details
Synonyms Pre-B Cell Colony Enhancing Factor 1; PBEF1; Nicotinamide Phosphoribosyltransferase
Product Type Monoclonal Antibody
Properties
Clone OMNI379
Isotype Mouse IgG2aκ
Immunogen/Antigen Recombinant human Nampt (visfatin/PBEF).
Application

ELISA: (direct or indirect: 1:2'000-1:10'000)
Flow Cytometry: (1:1'000)
Immunocytochemistry: (1:500-1:1’000)
Immunohistochemistry: (paraffin sections, 1:100-1:500)
Immunoprecipitation: (1:500-1:1’000)
Western blot: (1:2'000-1:5'000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature).
Optimal conditions should be determined individually for each application.
Crossreactivity Human
Mouse
Rat
Specificity

Recognizes human, mouse and rat Nampt.
Purity Detail Protein G-affinity purified.
Concentration 1mg/ml
Formulation Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Isotype Negative Control

Mouse IgG2a Isotype Control
RRID AB_2490117
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

Nicotinamide phosphoribosyltransferase (Nampt; pre-B cell colony-enhancing factor; PBEF; Visfatin) is an 52kDa adipokine secreted by adipose tissue and involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). Two forms of Nampt exist, an intracellular form (iNampt) and an extracellular form (eNampt). While the function of iNampt as an essential and rate-limiting NAD+ biosynthetic enzyme is well established, the physiological role of eNampt is still a matter of debate. Nampt has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.

Product References
  1. Hypoxic induction of human visfatin gene is directly mediated by hypoxia-inducible factor-1: S.K. Bae, et al.; FEBS Lett. 580, 4105 (2006)
  2. Molecular Characteristics of Serum Visfatin and Differential Detection by Immunoassays: A. Korner, et al.; J. Clin. Endocrinol. Metab. 92, 4783 (2007)
  3. Nampt/PBEF/Visfatin Regulates Insulin Secretion in beta Cells as a Systemic NAD Biosynthetic Enzyme: J.R. Revollo, et al.; Cell. Metab. 6, 363 (2007)
  4. Nicotinamide phosphoribosyltransferase (NAMPT/PBEF/visfatin) is constitutively released from human hepatocytes: A. Garten, et al.; BBRC 391, 376 (2010)
  5. Intracellular nicotinamide phosphoribosyltransferase protects against hepatocyte apoptosis and is down-regulated in nonalcoholic fatty liver disease: T.B. Dahl, et al.; J. Clin. Endocrinol. Metab. 95, 3039 (2010) Supplement Information
  6. Expression and regulation of nampt in human islets: K. Kover, et al.; PLoS One 8, e58767 (2013)
  7. Extracellular nicotinamide phosphoribosyltransferase (NAMPT) promotes M2 macrophage polarization in chronic lymphocytic leukemia: V. Audrito, et al.; Blood 125, 111 (2015)
  8. SIRT1-Mediated eNAMPT Secretion from Adipose Tissue Regulates Hypothalamic NAD+ and Function in Mice: M.J. Yoon, et al.; Cell Metab. 21, 706 (2015)
  9. Nicotinamide phosphoribosyltransferase production in human spermatozoa is influenced by maturation stage: S. Riammer, et al.; Andrology 4, 1045 (2016)
  10. Synthesis and degradation of adenosine 5'-tetraphosphate by nicotinamide and nicotinate phosphoribosyltransferases: A. Amici, et al.; Cell Chem. Biol. 24, 553 (2017)
  11. Nicotinamide phosphoribosyltransferase expression and clinical outcome of resected stage I/II pancreatic ductal adenocarcinoma: K. Davis, et al.; PLoS One 14, e0213576 (2019)
  12. Extracellular Vesicle-Contained eNAMPT Delays Aging and Extends Lifespan in Mice: M. Yoshida, et al.; Cell Metab. 30, 329 (2019)
  13. A nicotinamide phosphoribosyltransferase-GAPDH interaction sustains the stress-induced NMN/NAD+ salvage pathway in the nucleus: A.A. Grolla, et al.; J. Biol. Chem. 295, 3635 (2020)
  14. Human ESC-sEVs alleviate age-related bone loss by rejuvenating senescent bone marrow-derived mesenchymal stem cells: L. Gong, et al.; J. Extracell. Vesicles 9, 1800971 (2020)
  15. Tumors carrying BRAF-mutations over-express NAMPT that is genetically amplified and possesses oncogenic properties: V. Audrito, et al.; J. Transl. Med. 20, 118 (2022)
  16. Exercise increases the release of NAMPT in extracellular vesicles and alters NAD+ activity in recipient cells: M.C. Chong, et al.; Aging Cell 21, e13647 (2022)
  17. Extracellular nicotinamide phosphoribosyltransferase boosts IFNg-induced macrophage polarization independently of TLR4: G. Colombo, et al.; iScience 25, 104147 (2022)
  18. Pyruvate transamination and NAD biosynthesis enable proliferation of succinate dehydrogenase-deficient cells by supporting aerobic glycolysis: L. Ricci, et al.; Cell Death Dis. 14, 403 (2023)
  19. Identification of structural determinants of nicotinamide phosphoribosyl transferase (NAMPT) activity and substrate selectivity: D. Houry, et al.; J. Struct. Biol. 215, 108004 (2023)
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