AdipoGen Life Sciences

Nampt (Visfatin/PBEF) (mouse/rat) Dual ELISA Kit

CHF 660.00
In stock
AG-45A-0007YEK-KI0196 wellsCHF 660.00
AG-45A-0007YTP-KI012 x 96 wellsCHF 1'200.00
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Product Details
Synonyms Nicotinamide Phosphoribosyltransferase; PBEF1; Pre-B Cell Colony Enhancing Factor 1
Product Type Kit
Properties
Application Set Quantitative ELISA
Specificity Detects mouse and rat Nampt. Does not cross-react with human Nampt, mouse adiponectin, mouse resistin, mouse vaspin or mouse RBP4.
Crossreactivity Mouse
Rat
Quantity 1 x 96 wells
2 x 96 wells (Twin Plex)
Sensitivity 50pg/ml
Range 0.5 to 32ng/ml
Sample Type Serum
Assay Type Sandwich
Detection Type Colorimetric
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage +4°C
Handling Advice After standard reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Plate and reagents should reach room temperature before use.
Use/Stability 12 months after the day of manufacturing. See expiry date on ELISA Kit box.
Documents
Manual Download PDF
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

Nicotinamide phosphoribosyltransferase (Nampt; pre-B cell colony-enhancing factor; PBEF; Visfatin) is an 52kDA adipokine secreted by adipose tissue and involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). Two forms of Nampt exist, an intracellular form (iNampt) and an extracellular form (eNampt). While the function of iNampt as an essential and rate-limiting NAD+ biosynthetic enzyme is well established, the physiological role of eNampt is still a matter of debate. It has been shown that Nampt expression is increased in different diseases, like diabetes, obesity, fetal growth retardation, sepsis, inflammatory bowel disease, Crohn’s disease and rheumatoid arthritis. Furthermore, Nampt might be a marker of pre-eclampsia and of endothelial health. Recently NAMPT extracellular (eNAMPT) has been shown to be secreted from macrophages in muscles and to triggers a proliferative signal of muscle stem cell (MusC) by binding to CCR5 located at the surface receptor of MusC.

Product References
  1. Nampt secreted from cardiomyocytes promotes development of cardiac hypertrophy and adverse ventricular remodeling: V.B. Pillai, et al.; Am. J. Physiol. Heart Circ. Physiol. 304, H415 (2013)
  2. Hepatectomy-related hypophosphatemia: a novel phosphaturic factor in the liver-kidney axis: K. Nomura, et al.; J. Am. Soc. Nephrol. 25, 761 (2014)
  3. Nicotinamide phosphoribosyltransferase inhibitor is a novel therapeutic candidate in murine models of inflammatory lung injury: L. Moreno-Vinasco, et al.; Am. J. Respir. Cell Mol. Biol. 51, 223 (2014)
  4. Hepatic NAD+ deficiency as a therapeutic target for non-alcoholic fatty liver disease in ageing: C.-C. Zhou, et al.; Br. J. Pharmacol. 173, 2352 (2016)
  5. NAMPT inhibitor protects ischemic neuronal injury in rat brain via anti-neuroinflammation: C.-X. Chen, et al.; Neuroscience 356, 193 (2017)
  6. Neutralization of extracellular NAMPT (nicotinamide phosphoribosyltransferase) ameliorates experimental murine colitis: G. Colombo, et al.; J. Mol. Med. 98, 595 (2020)
  7. CD38 downregulation modulates NAD+ and NADP(H) levels in thermogenic adipose tissues: A. Benzi, et al.; BBA Mol. Cell Biol. Lipids 1866, 158819 (2021)
  8. NAMPT-targeting PROTAC promotes antitumor immunity via suppressing myeloid-derived suppressor cell expansion: Y. Wu, et al.; Acta Pharm. Sin. B. 12, 2859 (2022)
  9. Extracellular nicotinamide phosphoribosyltransferase boosts IFNg-induced macrophage polarization independently of TLR4: G. Colombo, et al.; iScience 25, 104147 (2022)
  10. Extracellular nicotinamide phosphoribosyltransferase (eNAMPT) neutralization counteracts T cell immune evasion in breast cancer: C. Travelli, et al.; J. Immunother. Cancer 11, e007010 (2023)
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