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AdipoGen Life Sciences
Caspase-1 (mouse) ELISA Kit
620
CHF
CHF 620.00
In stock
AG-45B-0002-KI0196 wellsCHF 620.00
Figure 1: Standard curve
| Product Details | |
|---|---|
| Synonyms | Interleukin-1β Convertase; IL-1BC; Interleukin-1β-converting Enzyme; ICE |
| Product Type | Kit |
| Properties | |
| Application Set | Quantitative ELISA |
| Specificity | Detects mouse caspase-1 (proform as well as p10 and p20 domain). Does not detect human caspase-1. |
| Crossreactivity | Mouse |
| Quantity | 1 x 96 wells |
| Sensitivity | 33pg/ml |
| Range | 0.15ng/ml to 10ng/ml |
| Sample Type |
Cell Culture Supernatant Plasma Serum |
| Assay Type | Sandwich |
| Detection Type | Colorimetric |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | +4°C |
| Handling Advice |
After standard reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Plate and reagents should reach room temperature before use. |
| Use/Stability | 12 months after the day of manufacturing. See expiry date on ELISA Kit box. |
| Documents | |
| Manual |
 Download PDF |
| MSDS |
Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Caspase-1 is the best-described inflammatory caspase. It processes the cytokines interleukin-1β (IL-1β) and interleukin-18 (IL-18) and induces pyroptotic cell death. Caspase-1 is activated by multiprotein complexes called inflammasomes in response to numerous stimuli that are detected through distinct inflammasomes. NLRC4 responds to cytosolic flagellin, murine NLRP1b responds to anthrax lethal toxin, AIM2 responds to cytosolic DNA and NLRP3/NALP3 responds to a variety of agonists including crystals.
This Caspase-1 (mouse) ELISA Kit can be used to measure caspase-1 (mouse) in cell culture supernatants, serum and plasma. It is a quantitative detection method, alternative to Western blotting to measure caspase-1 secretion.
Product References
- Pharmacologic inhibition of IκB kinase activates immediate hypersensitivity reactions in mice: D. Miyazaki, et al.; Am. J. Pathol. 183, 96 (2013)
- Alendronate augments lipid A-induced IL-1β release and Smad3/NLRP3/ASC-dependent cell death: R. Tamai & Y. Kiyoura; Life Sci. 198, 8 (2018)
- Inflammatory Monocytes Drive Influenza A Virus-Mediated Lung Injury in Juvenile Mice: B.M. Coates, et al.; J. Immunol. 200, 2391 (2018)
- Leishmania Lipophosphoglycan Triggers Caspase-11 and the Non-canonical Activation of the NLRP3 Inflammasome: R.V.H. de Carvalho, et al.; Cell Rep. 26, 429 (2019)
- High glutamate concentration reverses the inhibitory effect of microglial adenosine 2A receptor on NLRP3 inflammasome assembly and activation: H. Du, et al.; Neurosci. Lett. 769, 136431 (2022)
- CARD-only proteins regulate in vivo inflammasome responses and ameliorate gout: S. Devi, et al.; Cell Rep. 42, 112265 (2023)
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