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AdipoGen Life Sciences
Caspase-1 (mouse) Matched Pair Detection Set
750
CHF
CHF 750.00
In stock
AG-46B-0003-KI011 SetCHF 750.00
Reagents for 5 x 96-well plates
Figure 1: Mouse Caspase-1 is detected in cell culture supernatants by ELISA using Caspase-1 (mouse) Matched Pair Detection Set (Prod. No AG-46B-0003).
Method: Caspase-1 (mouse) was measured by ELISA in supernatants of differentiated bone marrow-derived dendritic cells (BMDCs) from wild-type (WT) or caspase-1-/- (KO) mice activated or not by 5 μM Nigericin (Nig) (AG-CN2-0020) for 30 min.
Method: Caspase-1 (mouse) was measured by ELISA in supernatants of differentiated bone marrow-derived dendritic cells (BMDCs) from wild-type (WT) or caspase-1-/- (KO) mice activated or not by 5 μM Nigericin (Nig) (AG-CN2-0020) for 30 min.
| Product Details | |
|---|---|
| Synonyms | Interleukin-1β Convertase; IL-1BC; Interleukin-1β-converting Enzyme; ICE |
| Product Type | Kit |
| Properties | |
| Application Set | Quantitative ELISA |
| Specificity | Detects mouse Caspase-1 (p10 and p20 domain). Does not detect human Caspase-1. |
| Crossreactivity | Mouse |
| Quantity | Contains sufficient materials to run ELISAs on 5 x 96-well plates. |
| Sensitivity | 50pg/ml |
| Range | 0.15ng/ml to 10ng/ml. |
| Sample Type | Cell Culture Supernatant |
| Assay Type | Sandwich |
| Detection Type | Colorimetric |
| Kit Contains |
1 vial standard protein (1μg) (lyophilized) [STD] 1 vial coating antibody (120μg) [COAT] 1 vial detection antibody (60μg) [DET] 1 vial streptavidin-HRP (10μg) (lyophilized) [STREP] |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | -20°C |
| Long Term Storage | -20°C |
| Handling Advice | Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| Manual |
 Download PDF |
| MSDS |
Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Caspase-1 is the best-described inflammatory caspase. It processes the cytokines interleukin-1β (IL-1β) and interleukin-18 (IL-18) and induces pyroptotic cell death. Caspase-1 is activated by multiprotein complexes called inflammasomes in response to numerous stimuli that are detected through distinct inflammasomes. NLRC4 responds to cytosolic flagellin, murine NLRP1b responds to anthrax lethal toxin, AIM2 responds to cytosolic DNA and NLRP3/NALP3 responds to a variety of agonists including crystals. The caspase-1 (mouse) Matched Pair Detection Set contains all reagents for the development of a sandwich ELISA to measure caspase-1 (mouse) in cell culture supernatants. This Matched Pair Detection Set is a quantitative detection method, alternative to Western blotting to measure caspase-1 secretion.
Product References
- ATG16L1 deficiency in macrophages drives clearance of uropathogenic E. coli in an IL-1b-dependent manner: J.W. Symington, et al.; Muc. Immunol. 8, 1388 (2015)
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