Caspase-1 (mouse) Matched Pair Detection Set

CHF 750.00
In stock
AG-46B-0003-KI011 SetCHF 750.00
Reagents for 5 x 96-well plates
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Product Details
Synonyms Interleukin-1β Convertase; IL-1BC; Interleukin-1β-converting Enzyme; ICE
Product Type Kit
Properties
Application Set Quantitative ELISA
Specificity Detects mouse Caspase-1 (p10 and p20 domain). Does not detect human Caspase-1.
Crossreactivity Mouse
Quantity Contains sufficient materials to run ELISAs on 5 x 96-well plates.
Sensitivity 50pg/ml
Range 0.15ng/ml to 10ng/ml.
Sample Type Cell Culture Supernatant
Assay Type Sandwich
Detection Type Colorimetric
Kit Contains 1 vial standard protein (1μg) (lyophilized) [STD]
1 vial coating antibody (120μg) [COAT]
1 vial detection antibody (60μg) [DET]
1 vial streptavidin-HRP (10μg) (lyophilized) [STREP]
Shipping and Handling
Shipping BLUE ICE
Short Term Storage -20°C
Long Term Storage -20°C
Handling Advice Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
Manual Download PDF
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

Caspase-1 is the best-described inflammatory caspase. It processes the cytokines interleukin-1β (IL-1β) and interleukin-18 (IL-18) and induces pyroptotic cell death. Caspase-1 is activated by multiprotein complexes called inflammasomes in response to numerous stimuli that are detected through distinct inflammasomes. NLRC4 responds to cytosolic flagellin, murine NLRP1b responds to anthrax lethal toxin, AIM2 responds to cytosolic DNA and NLRP3/NALP3 responds to a variety of agonists including crystals. The caspase-1 (mouse) Matched Pair Detection Set contains all reagents for the development of a sandwich ELISA to measure caspase-1 (mouse) in cell culture supernatants. This Matched Pair Detection Set is a quantitative detection method, alternative to Western blotting to measure caspase-1 secretion.

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