anti-Caspase-1 (p20) (mouse), mAb (Casper-1)

CHF 460.00
In stock
AG-20B-0042-C100100 µgCHF 460.00
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Product Details
Synonyms Interleukin-1β Convertase; IL-1BC; Interleukin-1β-converting Enzyme; ICE
Product Type Monoclonal Antibody
Properties
Clone Casper-1
Isotype Mouse IgG1
Source/Host Purified from concentrated hybridoma tissue culture supernatant.
Immunogen/Antigen Recombinant mouse caspase-1.
Application

Western Blot (see online protocol): (1μg/ml) (no need to precipitate the cell supernatant for the detection of caspase-1 (mouse) upon inflammasome activation)
Immunohistochemistry: (1:500; paraffin sections)
Immunoprecipitation: (1:200)

Crossreactivity Mouse
Specificity

Recognizes endogenous full-length and activated (p20 fragment) mouse caspase-1.

Purity ≥95% (SDS-PAGE)
Purity Detail Protein G-affinity purified.
Concentration 1mg/ml
Formulation Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
Protocols Download PDF
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

Caspase-1 is the best-described inflammatory caspase. It processes the cytokines interleukin-1β (IL-1β) and IL-18 and induces pyroptotic cell death. Caspase-1 is activated by multiprotein complexes called Inflammasomes in response to numerous stimuli that are detected through distinct inflammasomes. NLRC4 responds to cytosolic flagellin, murine NLRP1b responds to anthrax lethal toxin, AIM2 responds to cytosolic DNA and NLRP3 responds to a variety of agonists including crystals.

Product References
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  2. Inflammasome Activators Induce Interleukin-1alpha Secretion via Distinct Pathways with Differential Requirement for the Protease Function of Caspase-1: O. Gross, et al.; Immunity 36, 388 (2012)
  3. Omega-3 Fatty Acids Prevent Inflammation and Metabolic Disorder through Inhibition of NLRP3 Inflammasome Activation: Y. Yan, et al.; Immunity 38, 1154 (2013)
  4. LRRFIP2 negatively regulates NLRP3 inflammasome activation in macrophages by promoting Flightless-I-mediated caspase-1 inhibition: J. Jin, et al.; Nat. Commun. 4, 2075 (2013)
  5. iGLuc: a luciferase-based inflammasome and protease activity reporter: E. Bartok, et al.; Nat. Methods 10, 147 (2013)
  6. Receptor interacting protein kinase 2–mediated mitophagy regulates inflammasome activation during virus infection: C. Lupfer, et al.; Nat. Immunol. 14, 480 (2013)
  7. Activation of the NLRP3 inflammasome by IAV virulence protein PB1-F2 contributes to severe pathophysiology and disease: J.L. McAuley, et al.; PLoS Pathog. 9, e1003392 (2013)
  8. Caspase-1 activity affects AIM2 speck formation/stability through a negative feedback loop: C. Juruj, et al.; Front. Cell. Infect. Microbiol. 3, 14 (2013)
  9. Inflammasome Activation by Altered Proteostasis: J.N. Shin, et al.; J. Biol. Chem. 288, 35886 (2013)
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  11. Inflammasome activation and inhibition in primary murine bone marrow-derived cells, and assays for IL-1α, IL-1β, and caspase-1: K.s. Schneider, et al.; Methods Mol. Biol. 1040, 117 (2013)
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  16. The NLRP3 inflammasome is released as a particulate danger signal that amplifies the inflammatory response: A. Baroja-Mazo, et al.; Nat. Immunol. 15, 738 (2014)
  17. Caspase-1 activation by NLRP3 inflammasome dampens IL-33-dependent house dust mite-induced allergic lung inflammation: F. Madouri, et al.; J. Mol. Cell Biol. 7, 351 (2015)
  18. ZBP1/DAI is an innate sensor of influenza virus triggering the NLRP3 inflammasome and programmed cell death pathways: T. Kuriakose, et al.; Sci. Immunol. 1, aag2045 (2016)
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  20. IL-1beta and caspase-1 drive autoinflammatory disease independently of IL-1alpha or caspase-8 in a mouse model of familial mediterranean fever: D. Sharma, et al.; Am. J. Pathol. 187, 236 (2017)
  21. The DNA inflammasome in human myeloid cells is initiated by a STING-cell death program upstream of NLRP3: M.M. Gaidt, et al.; Cell 171, 1110 (2017)
  22. Inhibition of Dpp8/9 Activates the Nlrp1b Inflammasome: M.C. Okondo, et al.; Cell Chem. Biol. 25, 1 (2018)
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