Caspase-1 (mouse) ELISA Kit
|Synonyms||Interleukin-1β Convertase; IL-1BC; Interleukin-1β-converting Enzyme; ICE|
|Application Set||Quantitative ELISA|
|Specificity||Detects mouse caspase-1 (proform as well as p10 and p20 domain). Does not detect human caspase-1.|
|Quantity||1 x 96 wells|
|Range||0.15ng/ml to 10ng/ml|
Cell Culture Supernatant
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||+4°C|
After standard reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Plate and reagents should reach room temperature before use.
|Use/Stability||12 months after the day of manufacturing. See expiry date on ELISA Kit box.|
|Product Specification Sheet|
Caspase-1 is the best-described inflammatory caspase. It processes the cytokines interleukin-1β (IL-1β) and interleukin-18 (IL-18) and induces pyroptotic cell death. Caspase-1 is activated by multiprotein complexes called inflammasomes in response to numerous stimuli that are detected through distinct inflammasomes. NLRC4 responds to cytosolic flagellin, murine NLRP1b responds to anthrax lethal toxin, AIM2 responds to cytosolic DNA and NLRP3/NALP3 responds to a variety of agonists including crystals.
This Caspase-1 (mouse) ELISA Kit can be used to measure caspase-1 (mouse) in cell culture supernatants, serum and plasma. It is a quantitative detection method, alternative to Western blotting to measure caspase-1 secretion.
- Pharmacologic inhibition of IκB kinase activates immediate hypersensitivity reactions in mice: D. Miyazaki, et al.; Am. J. Pathol. 183, 96 (2013)
- Alendronate augments lipid A-induced IL-1β release and Smad3/NLRP3/ASC-dependent cell death: R. Tamai & Y. Kiyoura; Life Sci. 198, 8 (2018)
- Inflammatory Monocytes Drive Influenza A Virus-Mediated Lung Injury in Juvenile Mice: B.M. Coates, et al.; J. Immunol. 200, 2391 (2018)
- Leishmania Lipophosphoglycan Triggers Caspase-11 and the Non-canonical Activation of the NLRP3 Inflammasome: R.V.H. de Carvalho, et al.; Cell Rep. 26, 429 (2019)