anti-Caspase-1 (p10) (mouse), mAb (Casper-2)
|Synonyms||Interleukin-1β Convertase; IL-1BC; Interleukin-1β-converting Enzyme; ICE|
|Product Type||Monoclonal Antibody|
|Source/Host||Purified from concentrated hybridoma tissue culture supernatant.|
|Immunogen/Antigen||Recombinant mouse caspase-1.|
Western Blot (see online protocol): (1μg/ml) (no need to precipitate the cell supernatant for the detection of caspase-1 (mouse) upon inflammasome activation)
Recognizes endogenous full-length and activated (p10 fragment) mouse caspase-1.
|Purity Detail||Protein G-affinity purified.|
|Formulation||Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.|
|Isotype Negative Control|
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
|Use/Stability||Stable for at least 1 year after receipt when stored at -20°C.|
|Product Specification Sheet|
Caspase-1 is the best-described inflammatory caspase. It processes the cytokines interleukin-1β (IL-1β) and IL-18 and induces pyroptotic cell death. Caspase-1 is activated by multiprotein complexes called inflammasomes in response to numerous stimuli that are detected through distinct inflammasomes. NLRC4 responds to cytosolic flagellin, murine NLRP1b responds to anthrax lethal toxin, AIM2 responds to cytosolic DNA and NLRP3 responds to a variety of agonists including crystals.
- Measuring the inflammasome: O. Gross; Methods Mol. Biol. 844, 199 (2012)
- XIAP Restricts TNF- and RIP3-Dependent Cell Death and Inflammasome Activation: M. Yabal, et al.; Cell Rep. 7, 1796 (2014)
- AMP-activated protein kinase suppresses urate crystal-induced inflammation and transduces colchicine effects in macrophages: Y. Wang, et al.; Ann. Rheum. Dis. 75, 286 (2016)
- Bile acids control inflammation and metabolic disorder through inhibition of NLRP3 inflammasome: C. Guo, et al.; Immunity 45, 802 (2016)
- IL-18 is critical for MAIT cell IFN- responses to Francisella 1 species in vitro but not in vivo: E. Jesteadt, et al.; Infect. Immun. 86, e00117 (2018)
- Interleukin-18 Is Critical for Mucosa-Associated Invariant T Cell Gamma Interferon Responses to Francisella Species In Vitro but Not In Vivo: E. Jesteadt, et al.; Infect. Immun. 86, e00117 (2018)