anti-Caspase-1 (p10) (mouse), mAb (Casper-2)

CHF 460.00
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AG-20B-0044-C100100 µgCHF 460.00
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Product Details
Synonyms Interleukin-1β Convertase; IL-1BC; Interleukin-1β-converting Enzyme; ICE
Product Type Monoclonal Antibody
Properties
Clone Casper-2
Isotype Mouse IgG2a
Source/Host Purified from concentrated hybridoma tissue culture supernatant.
Immunogen/Antigen Recombinant mouse caspase-1.
Application Western Blot (see online protocol): (1μg/ml) (no need to precipitate the cell supernatant for the detection of caspase-1 (mouse) upon inflammasome activation)
Crossreactivity Mouse
Specificity Recognizes endogenous full-length and activated (p10 fragment) mouse caspase-1.
Purity ≥95% (SDS-PAGE)
Purity Detail Protein G-affinity purified.
Concentration 1mg/ml
Formulation Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
Protocols Download PDF
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

Caspase-1 is the best-described inflammatory caspase. It processes the cytokines interleukin-1β (IL-1β) and IL-18 and induces pyroptotic cell death. Caspase-1 is activated by multiprotein complexes called inflammasomes in response to numerous stimuli that are detected through distinct inflammasomes. NLRC4 responds to cytosolic flagellin, murine NLRP1b responds to anthrax lethal toxin, AIM2 responds to cytosolic DNA and NLRP3 responds to a variety of agonists including crystals.

Product References
  1. Measuring the inflammasome: O. Gross; Methods Mol. Biol. 844, 199 (2012)
  2. XIAP Restricts TNF- and RIP3-Dependent Cell Death and Inflammasome Activation: M. Yabal, et al.; Cell Rep. 7, 1796 (2014)
  3. AMP-activated protein kinase suppresses urate crystal-induced inflammation and transduces colchicine effects in macrophages: Y. Wang, et al.; Ann. Rheum. Dis. 75, 286 (2016)
  4. Bile acids control inflammation and metabolic disorder through inhibition of NLRP3 inflammasome: C. Guo, et al.; Immunity 45, 802 (2016)
  5. IL-18 is critical for MAIT cell IFN- responses to Francisella 1 species in vitro but not in vivo: E. Jesteadt, et al.; Infect. Immun., ahead of print (2018)
  6. Interleukin-18 Is Critical for Mucosa-Associated Invariant T Cell Gamma Interferon Responses to Francisella Species In Vitro but Not In Vivo: E. Jesteadt, et al.; Infect. Immun. 86, e00117 (2018)
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