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AdipoGen Life Sciences
Monosodium urate (crystals)
105
CHF
CHF 105.00
In stock
AG-CR1-3950-M0022 mgCHF 105.00
AG-CR1-3950-20022 x 2 mgCHF 170.00
Activation of NLRP3/NALP3 with uric acid solution (MSU) (Prod. No. AG-CR1-3951).
Method: THP1 cells or THP1 expressing a shRNA directed to human NLRP3/NALP3 sequence were incubated with MSU (300µg /ml) for 6 hours. IL-1β secreted was measured with a IL-1β (human) ELISA kit.
Method: THP1 cells or THP1 expressing a shRNA directed to human NLRP3/NALP3 sequence were incubated with MSU (300µg /ml) for 6 hours. IL-1β secreted was measured with a IL-1β (human) ELISA kit.
| Product Details | |
|---|---|
| Synonyms | MSU (crystals); Uric acid (crystals) |
| Product Type | Chemical |
| Properties | |
| Formula |
C5H3N4O3 . Na |
| MW | 167.1 . 23.0 |
| CAS | 1198-77-2 |
| RTECS | YU7062000 |
| Appearance | White solid crystals. Solution may appear as a slightly hazy suspension. |
| Solubility | Not soluble in PBS. Reconstitute 2mg of MSU in 100μl PBS to obtain a 20mg/ml suspension. |
| Endotoxin Content | <0.001EU/μg purified protein (LAL test; Lonza). |
| InChi Key | NAFSTSRULRIERK-UHFFFAOYSA-M |
| Smiles | [Na+].[O-]C1=NC(=O)NC2=C1NC(=O)N2 |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Vortex before use. |
| Use/Stability |
Stable for at least 2 years after receipt when stored at -20°C. Working aliquots are stable for up to 12 months when stored at -20°C. |
| Documents | |
| Protocols |
 Download PDF |
| MSDS |
Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
- NLRP3/NALP3 inflammasome activator. Specially crystallized and tested for biological activity.
- Stimulates the caspase-1 activating NLRP3/NALP3 inflammasome, resulting in the production of active IL-1β and IL-18.
Product References
- Gout-associated uric acid crystals activate the NALP3 inflammasome: F. Martinon, et al.; Nature 440, 237 (2006)
- Malarial hemozoin activates the NLRP3 inflammasome through Lyn and Syk kinases: M.T. Shio, et al.; PLoS Pathog. 5, e1000559 (2009)
- Crystals of monosodium urate monohydrate enhance lipopolysaccharide-induced release of interleukin 1 beta by mononuclear cells through a caspase 1-mediated process: E.J. Giamarellos-Bourboulis, et al.; Ann. Rheum. Dis. 68, 273 (2009)
- Monosodium urate crystals in inflammation and immunity: Y. Shi, et al.; Immunol. Rev. 233, 203 (2010)
- RIP3-mediated necrotic cell death accelerates systematic inflammation and mortality: L. Meng, et al.; PNAS 112, 11007 (2015)
- Shigella hijacks the glomulin-cIAPs-inflammasome axis to promote inflammation: S. Suzuki, et al.; EMBO Rep.19, 89 (2018)
- Calcium phosphate particles stimulate interleukin-1β release from human vascular smooth muscle cells: A role for spleen tyrosine kinase and exosome release: Y. Dautova, et al.; J. Mol. Cell Cardiol. 115, 82 (2018)
- Cutting Edge: G Protein Subunit β 1 Negatively Regulates NLRP3 Inflammasome Activation: T. Murakami, et al.; J. Immunol. 202, 1942 (2019)
- Expression of a PYCARD/ASC variant lacking exon 2 in Japanese patients with palindromic rheumatism increases interleukin-1β secretion: Y. Suganuma, et al.; Asian Pac. J. Allergy Immunol. (Epub ahead of print) (2019)
- L-plastin enhances NLRP3 inflammasome assembly and bleomycin-induced lung fibrosis: H. Joshi, et al.; Cell Rep. 38, 110507 (2022)
- Urolithin A suppresses NLRP3 inflammasome activation by inhibiting the generation of reactive oxygen species and prevents monosodium urate crystal-induced peritonitis: W. Komatsu, et al. Biosci., Biotech., Biochem. 88, 966 (2024)
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