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AdipoGen Life Sciences
Monosodium urate (ready-to-use)
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490
CHF
CHF 490.00
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AG-CR1-3951-M01010 mgCHF 490.00
Activation of NLRP3/NALP3 with uric acid solution (MSU) (Prod. No. AG-CR1-3951).
Method: THP1 cells or THP1 expressing a shRNA directed to human NLRP3/NALP3 sequence were incubated with MSU (300µg /ml) for 6 hours. IL-1β secreted was measured with a IL-1β (human) ELISA kit.
Method: THP1 cells or THP1 expressing a shRNA directed to human NLRP3/NALP3 sequence were incubated with MSU (300µg /ml) for 6 hours. IL-1β secreted was measured with a IL-1β (human) ELISA kit.
Specifications / Handling
| Product Details | |
|---|---|
| Synonyms | MSU (ready-to-use); Uric acid (ready-to-use) |
| Product Type | Chemical |
| Properties | |
| Formula |
C5H3N4O3 . Na |
| MW | 167.1 . 23.0 |
| CAS | 1198-77-2 |
| RTECS | YU7062000 |
| Appearance | Liquid. Solution may appear as a slightly hazy suspension. |
| Formulation | In PBS. |
| Concentration | 20 mg/ml. |
| Endotoxin Content | <0.001EU/μg purified protein (LAL test; Lonza). |
| Other Product Data |
Note: Be sure to tap vials before opening to recover all liquid. |
| InChi Key | NAFSTSRULRIERK-UHFFFAOYSA-M |
| Smiles | [Na+].[O-]C1=NC(=O)NC2=C1NC(=O)N2 |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Vortex before use. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| Protocols |
 Download PDF |
| MSDS |
Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Scientific Background Information
Product Description
- NLRP3/NALP3 inflammasome activator. Specially crystallized and tested for biological activity.
- Stimulates the caspase-1 activating NLRP3/NALP3 inflammasome, resulting in the production of active IL-1β and IL-18.
Product-specific References
- Gout-associated uric acid crystals activate the NALP3 inflammasome: F. Martinon, et al.; Nature 440, 237 (2006) [PMID: 16407889]
- Malarial hemozoin activates the NLRP3 inflammasome through Lyn and Syk kinases: M.T. Shio, et al.; PLoS Pathog. 5, e1000559 (2009) [PMC2722371]
- Crystals of monosodium urate monohydrate enhance lipopolysaccharide-induced release of interleukin 1 beta by mononuclear cells through a caspase 1-mediated process: E.J. Giamarellos-Bourboulis, et al.; Ann. Rheum. Dis. 68, 273 (2009) [PMID: 18390571]
- Monosodium urate crystals in inflammation and immunity: Y. Shi, et al.; Immunol. Rev. 233, 203 (2010) [PMID: 20193001]
- Calcium phosphate particles stimulate interleukin-1β release from human vascular smooth muscle cells: A role for spleen tyrosine kinase and exosome release: Y. Dautova, et al.; J. Mol. Cell Cardiol. 115, 82 (2018) [PMC5823844]
- Cutting Edge: G Protein Subunit β 1 Negatively Regulates NLRP3 Inflammasome Activation: T. Murakami, et al.; J. Immunol. 202, 1942 (2019) [PMID: 30777924]
- Expression of a PYCARD/ASC variant lacking exon 2 in Japanese patients with palindromic rheumatism increases interleukin-1β secretion: Y. Suganuma, et al.; Asian Pac. J. Allergy Immunol. 40, 81 (2019) [PMID: 31421660]
- Urolithin A suppresses NLRP3 inflammasome activation by inhibiting the generation of reactive oxygen species and prevents monosodium urate crystal-induced peritonitis: W. Komatsu, et al. Biosci., Biotech., Biochem. 88, 966 (2024) [PMID: 38772744]
Additional References using AdipoGen AG-CR1-3950/AG-CR1-3951: - RIP3-mediated necrotic cell death accelerates systematic inflammation and mortality: L. Meng, et al.; PNAS 112, 11007 (2015) [PMC4568286]
- IL-1 beta but not the NALP3 inflammasome is an important determinant of endothelial cell responses to necrotic/dangerous trophoblastic debris: J. Wei, et al.; Placenta 36, 1385 (2015) [PMID: 26515928]
- Shigella hijacks the glomulin-cIAPs-inflammasome axis to promote inflammation: S. Suzuki, et al.; EMBO Rep.19, 89 (2018) [PMC5757219]
- Optimized protocols for studying the NLRP3 inflammasome and assessment of potential targets of CP-453,773 in undifferentiated THP1 cells: J.A. Guzova, et al.; J. Immunol. Methods 467, 19 (2019) [PMID: 30735689]
- L-plastin enhances NLRP3 inflammasome assembly and bleomycin-induced lung fibrosis: H. Joshi, et al.; Cell Rep. 38, 110507 (2022) [PMC8998782]
- SERTAD1 initiates NLRP3-mediated inflammasome activation through restricting NLRP3 polyubiquitination: J. Ha, et al.; Cell Rep. 43, 113752 (2024) [PMID: 38341852]
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