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Chemodex
5(6)-CFDA
Product Details | |
---|---|
Synonyms | 5(6)-Carboxyfluorescein diacetate |
Product Type | Chemical |
Properties | |
Formula | C25H16O9 |
MW | 460.39 |
CAS | 124387-19-5 |
Source/Host Chemicals | Synthetic. |
Purity Chemicals | ≥90% (HPLC) |
Appearance | White powder. |
Solubility | Soluble in DMSO. |
Identity | Determined by 1H-NMR. |
Declaration | Manufactured by Chemodex. |
Other Product Data |
Click here for Original Manufacturer Product Datasheet |
InChi Key | DCPDRLHHNBUQPJ-UHFFFAOYSA-N |
Smiles | CC(O)=O.CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC=C3)C1=CC=C(OC(C)=O)C=C1O2 |
Shipping and Handling | |
Shipping | AMBIENT |
Short Term Storage | +4°C |
Long Term Storage | +4°C |
Handling Advice | Protect from light and moisture. |
Use/Stability | Stable for at least 2 years after receipt when stored at +4°C. |
Documents | |
MSDS | Download PDF |
Product Specification Sheet | |
Datasheet | Download PDF |
CFDA is a popular, sensitive, fluorescent substrate for measuring esterase activity in live cells. It is often used in cell viability assays and "Live-Dead" assay systems, especially automated cell viability assays. The cleavage product produced upon enzymatic or chemical hydrolysis of the acetate group(s) is carboxyfluorescein which is well-retained inside live cells, while the substrate CFDA is membrane permeant.
(1) C. Thrane et al.; J. Microbiol.l Meth. 28(1), 35 (1997) | (2) G. Liminga et al.; Anti-Cancer Drugs 6(4), 578 (1995) | (3) L.S. DeClerck et al.; J. Immun. Meth. 172(1), 115 (1994)