CHF 71.00
In stock
CDX-C0039-M02525 mgCHF 71.00
CDX-C0039-M100100 mgCHF 206.00
CDX-C0039-G0011 gCHF 1'373.00
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Product Details
Synonyms 5-Carboxyfluorescein diacetate
Product Type Chemical


MW 460.39
CAS 79955-27-4
Source/Host Chemicals Synthetic.
Purity Chemicals ≥95% (HPLC)
Appearance White to yellow solid.
Solubility Soluble in DMSO.
Identity Determined by 1H-NMR.
Declaration Manufactured by Chemodex.
Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

Smiles CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC(=C3)C(O)=O)C1=CC=C(OC(C)=O)C=C1O2
Shipping and Handling
Shipping AMBIENT
Short Term Storage +4°C
Long Term Storage +4°C
Handling Advice Protect from light and moisture.
Use/Stability Stable for at least 2 years after receipt when stored at +4°C.
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

Carboxyfluorescein diacetate (CFDA), which was originally used to measure intracellular pH but was soon adapted for use as a cell viability indicator. Upon hydrolysis by intracellular nonspecific esterases, CFDA forms carboxyfluorescein. As compared with fluorescein, carboxyfluorescein contains extra negative charges and is therefore better retained in cells CFDA is moderately permeant to most cell membranes, with uptake greater at pH 6.2 than at pH 7.41. CFDA has been used as a viability probe with a variety of cells, including bacteria, fungi (e.g., Saccharomyces cerevisiae), spermatozoa, natural killer (NK) cells and tumor cells.

Product References

(1) J. Immun. Methods 172, 115 (1994) | (2) Anticancer Res. 14, 927 (1994) | (3) Appl. Environ Microbiol. 60, 1467 (1994) | (4) Cytometry 15, 213 (1994) | (5) Biol. Reprod. 34, 127 (1986) | (6) Immun. Lett. 2, 187 (1981)

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