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Chemodex
5-CFDA N-succinimidyl ester
131
CHF
CHF 131.00
In stock
CDX-C0094-M0055 mgCHF 131.00
CDX-C0094-M02525 mgCHF 326.00
CDX-C0094-M100100 mgCHF 1'004.00
Product Details | |
---|---|
Synonyms | 5-Carboxyfluorescein diacetate N-succinimidyl ester |
Product Type | Chemical |
Properties | |
Formula |
C29H19NO11 |
MW | 557.46 |
CAS | 150206-05-6 |
Source/Host Chemicals | Synthetic. |
Purity Chemicals | ≥95% (HPLC) |
Appearance | White to faint yellow powder. |
Solubility | Soluble in DMSO or DMF. |
Identity | Determined by 1H-NMR. |
Declaration | Manufactured by Chemodex. |
Other Product Data |
Click here for Original Manufacturer Product Datasheet |
InChi Key | SRSXVRUMXPCNAJ-UHFFFAOYSA-N |
Smiles | CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC(=C3)C(=O)ON1C(=O)CCC1=O)C1=CC=C(OC(C)=O)C=C1O2 |
Shipping and Handling | |
Shipping | AMBIENT |
Short Term Storage | +4°C |
Long Term Storage | -20°C |
Handling Advice | Protect from light and moisture. |
Use/Stability | Stable for at least 2 years after receipt when stored at -20°C. |
Documents | |
MSDS | Download PDF |
Product Specification Sheet | |
Datasheet | Download PDF |
Description
5-CFDA is membrane-permeant and can be loaded into cells via incubation. Once inside the cells, 5-CFDA is hydrolyzed by intracellular esterases to 5-carboxyfluorescein. It has been used for labeling human intervertebral disk cells in vitro for fluorescence microscopy. Continuous determination of the intracellular pH in bacteria by fluorometry.
Product References
(1) G.O. Bakhus. Et al.; Immunologiya 23(3), 190 (2002) | (2) H.E. Gruber et al.; Biotech. Histochem. 75, 118 (2000) | (3) P. Breeuwer et al.; Appl. Environ. Microbiol. 62, 178 (1996) | (4) S. Boitano et al.; J. Cell Sci. 98, 343 (1991)