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Chemodex
5(6)-CFDA N-succinimidyl ester
Specifications / Handling
| Product Details | |
|---|---|
| Synonyms | 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester |
| Product Type | Chemical |
| Properties | |
| Formula | C29H19NO11 |
| MW | 557.46 |
| CAS | 150347-59-4 |
| Source/Host Chemicals | Synthetic. |
| Purity Chemicals | ≥90% (HPLC) |
| Appearance | White or slightly yellow powder. |
| Solubility | Soluble in DMSO or DMF. |
| Identity | Determined by 1H-NMR. |
| Declaration | Manufactured by Chemodex. |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| InChi Key | KQPBJOQXHVRGEG-UHFFFAOYSA-N |
| Smiles | CC(=O)ON1C(=O)CCC1=O.CC(=O)OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC=C3)C1=CC=C(OC(C)=O)C=C1O2 |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use/Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Documents | |
| Safety Data Sheet |
Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Scientific Background Information
5(6)-Carboxyfluorescein diacetate N-succinimidyl ester is a useful fluorescent tracer that can passivley diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling. The reagent itself is colorless and nonfluorescent but becomes brightly green fluorescent once it is hydrolyzed by intracellular esterases.
(1) V.V. Ganusov et al.; J. Immun. Meth. 298(1-2), 183 (2005) | (2) C. Bergsdorf et al.; FEBS Letters 536(1-3), 120 (2003) | (3) I.E. Dumitriu et al.; Analyt. Biochem. 299, 247 (2001) | (4) A. Lyons et al.; Meth. Cell Biol. 63, 375 (2001)
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