Cookie Policy: This site uses cookies to improve your experience. You can find out more about our use of cookies in our Privacy Policy. By continuing to browse this site you agree to our use of cookies.
AdipoGen Life Sciences
anti-DLK1 (human), mAb (PF299-1)
300
CHF
CHF 300.00
In stock
AG-20A-0070-C05050 µgCHF 300.00
AG-20A-0070-C100100 µgCHF 390.00
Figure 1: Immunohistochemical staining of DLK1 using anti-DLK1 (human), mAb (PF299-1) (Prod. No. AG-20A-0070) in placenta, villi (1:500 dilution).
This antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
This antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
Figure 2: FACS: DLK1-transfected HEK 293 (Full length) cells were stained significantly using anti-DLK1 (human), mAb (PF299-1) (Prod. No. AG-20A-0070).
Figure 3: Western blot analysis of human DLK1 using anti-DLK1 (human), mAb (PF299-1) (Prod. No. AG-20A-0070) at 1: 2,000 dilution.
A.
1. Human DLK1-Fc protein.
2. Transfected human DLK1 full length cell lysate (HEK 293).
3. Mock Transfected HEK293 cell lysate.
B.
1. Human DLK1-Fc protein.
2. Mouse DLK1-Fc protein.
3. Human DNER-Fc protein.
4. Human DLL1-Fc protein.
5. Human DLL3-Fc protein.
6. Human-DLL4 Fc protein.
7. Human Jagged1-Fc protein.
8. Human FGF23-Fc protein.
A.
1. Human DLK1-Fc protein.
2. Transfected human DLK1 full length cell lysate (HEK 293).
3. Mock Transfected HEK293 cell lysate.
B.
1. Human DLK1-Fc protein.
2. Mouse DLK1-Fc protein.
3. Human DNER-Fc protein.
4. Human DLL1-Fc protein.
5. Human DLL3-Fc protein.
6. Human-DLL4 Fc protein.
7. Human Jagged1-Fc protein.
8. Human FGF23-Fc protein.
| Product Details | |
|---|---|
| Synonyms | Protein Delta Homolog 1; Fetal Antigen 1; Preadipocyte Factor 1; Pref-1; FA1 |
| Product Type | Monoclonal Antibody |
| Properties | |
| Clone | PF299-1 |
| Isotype | Mouse IgG1κ |
| Immunogen/Antigen | Recombinant human DLK1 (extracellular domain). |
| Application |
ELISA: (direct or indirect: 1:2'000-1:10'000) |
| Crossreactivity | Human |
| Specificity |
Recognizes human DLK1. Does not cross-react with mouse DLK1. |
| Purity Detail | Protein G-affinity purified. |
| Concentration | 1mg/ml |
| Formulation | Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives. |
| Isotype Negative Control | |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
The transmembrane and secreted protein delta-like 1 homolog (DLK1; Protein delta homolog 1; Preadipocyte factor 1; Pref-1) belongs to the EGF-like family. It can be cleaved by a tumor necrosis factor-α converting enzyme (TACE) to release a soluble form. DLK1 plays important roles in regulating cell differentiation such as adipogenesis and osteogenesis. Membrane-bound DLK1 promotes hypertrophic myotube formation, whereas soluble DLK1 inhibits myotube formation. The soluble form of DLK1 inhibits preadipocyte differentiation, while membrane DLK1 represses preadipocyte proliferation. DLK1 is also associated with tumor invasion.
Product References
- Increased Haematopoietic Supportive Function of USSC from Umbilical Cord Blood Compared to CB MSC and Possible Role of DLK-1: S.M. Kluth, et al.; Stem Cells Int. 2013, ID985285 (2013)
- A comparative analysis of the adipogenic potential in human mesenchymal stromal cells from cord blood and other sources: M. Karagianni, et al.; Cytotherapy 15, 76 (2013)
- DLK1: A Novel Target for Immunotherapeutic Remodeling of the Tumor Blood Vasculature: N.C. Sabins, et al.; Mol. Ther. 21, 1958 (2013)
- A proteogenomic surfaceome study identifies DLK1 as an immunotherapeutic target in neuroblastoma: A.K. Hamilton, et al.; Cancer Cell (ePub ahead) (2024)
- Identification of DLK1, a Notch ligand, as an immunotherapeutic target and regulator of 1 tumor cell plasticity and chemoresistance in adrenocortical carcinoma: N.-Y. Sun, et al.; bioRxiv (ePub ahead) (2024)
Related Products
