AdipoGen Life Sciences

anti-DLK1 (human), mAb (PF13-3)

CHF 300.00
In stock
AG-20A-0069-C05050 µgCHF 300.00
AG-20A-0069-C100100 µgCHF 390.00
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Product Details
Synonyms Protein Delta Homolog 1; Fetal Antigen 1; Preadipocyte Factor 1; Pref-1; FA1
Product Type Monoclonal Antibody
Properties
Clone PF13-3
Isotype Mouse IgG1κ
Immunogen/Antigen Recombinant human DLK1 (extracellular domain).
Application

ELISA: (direct or indirect: 1:2'000-1:10'000)
Flow Cytometry: (1:100-1:500)
Immunohistochemistry: (10μg/ml)
Western blot: (1:2'000-1:10'000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature).
Optimal conditions should be determined individually for each application.

Crossreactivity Human
Specificity

Recognizes human DLK1. Does not cross-react with mouse DLK1.

Purity Detail Protein G-affinity purified.
Concentration 1mg/ml
Formulation Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Isotype Negative Control

Mouse IgG1 Isotype Control

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

The transmembrane and secreted protein delta-like 1 homolog (DLK1; Protein delta homolog 1; Preadipocyte factor 1; Pref-1) belongs to the EGF-like family. It can be cleaved by a tumor necrosis factor-α converting enzyme (TACE) to release a soluble form. DLK1 plays important roles in regulating cell differentiation such as adipogenesis and osteogenesis. Membrane-bound DLK1 promotes hypertrophic myotube formation, whereas soluble DLK1 inhibits myotube formation. The soluble form of DLK1 inhibits preadipocyte differentiation, while membrane DLK1 represses preadipocyte proliferation. DLK1 is also associated with tumor invasion.

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