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AdipoGen Life Sciences
anti-DLK1 (human), pAb
320
CHF
CHF 320.00
In stock
AG-25A-0091-C100100 µgCHF 320.00
Figure 1: FACS: DLK1 transfected HEK 293 cells were stained significantly using anti-DLK1 (human), pAb (Prod. No. AG-25A-0091).
Figure 2: Western blot analysis of human DLK1 using anti-DLK1 (human), pAb (Prod. No. AG-25A-0091) at 1: 2,000 dilution.
A.
1. Human DLK1 (Fc protein).
2. Transfected human DLK1 full length cell lysate (HEK 293).
3. Mock Transfected HEK293 cell lysate.
B.
1. Human DLK-1 (Fc protein), 200ng.
2. Human DLK-1(ecd) (FLAG-tagged), 200ng.
3. Human DLL1(ecd) (FLAG-tagged), 200ng.
4. Mouse Vaspin (Fc protein) (control), 200ng.
5. Human Klotho (Fc protein) (control), 200ng.
6. Human Jagged1 (Fc protein) (control), 200ng.
A.
1. Human DLK1 (Fc protein).
2. Transfected human DLK1 full length cell lysate (HEK 293).
3. Mock Transfected HEK293 cell lysate.
B.
1. Human DLK-1 (Fc protein), 200ng.
2. Human DLK-1(ecd) (FLAG-tagged), 200ng.
3. Human DLL1(ecd) (FLAG-tagged), 200ng.
4. Mouse Vaspin (Fc protein) (control), 200ng.
5. Human Klotho (Fc protein) (control), 200ng.
6. Human Jagged1 (Fc protein) (control), 200ng.
| Product Details | |
|---|---|
| Synonyms | Protein Delta Homolog 1; Fetal Antigen 1; Preadipocyte Factor 1; Pref-1; FA1 |
| Product Type | Polyclonal Antibody |
| Properties | |
| Source/Host | Rat |
| Immunogen/Antigen | Recombinant human DLK1 (extracellular domain). |
| Application |
ELISA: (direct and indirect: 1:2’000-1:10’000) |
| Crossreactivity | Human |
| Specificity |
Recognizes human DLK1. Detects a band of ~42kDa by Western blot. |
| Purity Detail | Protein A-affinity purified. |
| Concentration | 1mg/ml |
| Formulation | Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 6 months after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
The transmembrane and secreted protein delta-like 1 homolog (DLK1; Protein delta homolog 1; Preadipocyte factor 1; Pref-1) belongs to the EGF-like family. It can be cleaved by a tumor necrosis factor-α converting enzyme (TACE) to release a soluble form. DLK1 plays important roles in regulating cell differentiation such as adipogenesis and osteogenesis. Membrane-bound DLK1 promotes hypertrophic myotube formation, whereas soluble DLK1 inhibits myotube formation. The soluble form of DLK1 inhibits preadipocyte differentiation, while membrane DLK1 represses preadipocyte proliferation. DLK1 is also associated with tumor invasion.
Product References
- DLK1(PREF1) is a negative regulator of adipogenesis in CD105⁺/CD90⁺/CD34⁺/CD31⁻/FABP4⁻ adipose-derived stromal cells from subcutaneous abdominal fat pats of adult women: M.C. Mitterberger, et al.; Stem Cell Res. 9, 35 (2012) [KO validation]
- Reliable reference genes for expression analysis of proliferating and adipogenically differentiating human adipose stromal cells: C. Krautgasser, et al.; Cell. Mol. Biol. Lett. 24, 14 (2019)
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