AdipoGen Life Sciences

anti-DLK1 (human), pAb

CHF 320.00
In stock
AG-25A-0091-C100100 µgCHF 320.00
More Information
Product Details
Synonyms Protein Delta Homolog 1; Fetal Antigen 1; Preadipocyte Factor 1; Pref-1; FA1
Product Type Polyclonal Antibody
Properties
Source/Host Rat
Immunogen/Antigen Recombinant human DLK1 (extracellular domain).
Application

ELISA: (direct and indirect: 1:2’000-1:10’000)
Flow Cytometry: (1:100-1:500)
Western Blot: (1:2’000-1:10’000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature).
Optimal conditions must be determined individually for each application.

Crossreactivity Human
Specificity

Recognizes human DLK1. Detects a band of ~42kDa by Western blot.

Purity Detail Protein A-affinity purified.
Concentration 1mg/ml
Formulation Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

The transmembrane and secreted protein delta-like 1 homolog (DLK1; Protein delta homolog 1; Preadipocyte factor 1; Pref-1) belongs to the EGF-like family. It can be cleaved by a tumor necrosis factor-α converting enzyme (TACE) to release a soluble form. DLK1 plays important roles in regulating cell differentiation such as adipogenesis and osteogenesis. Membrane-bound DLK1 promotes hypertrophic myotube formation, whereas soluble DLK1 inhibits myotube formation. The soluble form of DLK1 inhibits preadipocyte differentiation, while membrane DLK1 represses preadipocyte proliferation. DLK1 is also associated with tumor invasion.

Product References
  1. DLK1(PREF1) is a negative regulator of adipogenesis in CD105⁺/CD90⁺/CD34⁺/CD31⁻/FABP4⁻ adipose-derived stromal cells from subcutaneous abdominal fat pats of adult women: M.C. Mitterberger, et al.; Stem Cell Res. 9, 35 (2012) [KO validation]
  2. Reliable reference genes for expression analysis of proliferating and adipogenically differentiating human adipose stromal cells: C. Krautgasser, et al.; Cell. Mol. Biol. Lett. 24, 14 (2019)
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