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AdipoGen Life Sciences
anti-DLK1 (human), pAb
190
CHF
CHF 190.00
In stock
AG-25A-0092-C100100 µgCHF 190.00
Figure 1: Immunohistochemical staining of DLK1 using anti-DLK1 (human), pAb (Prod. No. AG-25A-0092) in human adrenal tissue (5µg/ml).
This antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
This antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
Figure 2: Western blot analysis using anti-DLK1 (human), pAb (Prod. No. AG-25A-0092) at 1:5'000 dilution.
1: Human DLK1-Fc protein.
2: Human DLK1 (ED) (FLAG®-tagged).
3: Mouse DLK1-Fc protein.
4: Human DLL1-Fc protein.
5: Human DLL3-Fc protein.
6: Human DLK4-Fc protein.
7: Human Jagged-1-Fc protein.
8: Human DNER-Fc protein.
9: Human ACE2-Fc protein.
1: Human DLK1-Fc protein.
2: Human DLK1 (ED) (FLAG®-tagged).
3: Mouse DLK1-Fc protein.
4: Human DLL1-Fc protein.
5: Human DLL3-Fc protein.
6: Human DLK4-Fc protein.
7: Human Jagged-1-Fc protein.
8: Human DNER-Fc protein.
9: Human ACE2-Fc protein.
| Product Details | |
|---|---|
| Synonyms | Protein Delta Homolog 1; Fetal Antigen 1; Preadipocyte Factor 1; Pref-1; FA1 |
| Product Type | Polyclonal Antibody |
| Properties | |
| Source/Host | Rabbit |
| Immunogen/Antigen | Recombinant human DLK1 (extracellular domain). |
| Application |
ELISA: (direct and indirect: 1:2’000-1:10’000) Note: Tested on recombinant proteins and/or target-protein transfected cell lines in ELISA, Western Blot and/or FACS. |
| Crossreactivity | Human |
| Specificity |
Recognizes human DLK1. Detects a band of ~42kDa by Western blot. Weakly cross-reacts with mouse Dlk1. |
| Purity Detail | Protein A-affinity purified. |
| Concentration | 1mg/ml |
| Formulation | Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives. |
| Isotype Negative Control | |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 6 months after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
The transmembrane and secreted protein delta-like 1 homolog (DLK1; Protein delta homolog 1; Preadipocyte factor 1; Pref-1) belongs to the EGF-like family. It can be cleaved by a tumor necrosis factor-α converting enzyme (TACE) to release a soluble form. DLK1 plays important roles in regulating cell differentiation such as adipogenesis and osteogenesis. Membrane-bound DLK1 promotes hypertrophic myotube formation, whereas soluble DLK1 inhibits myotube formation. The soluble form of DLK1 inhibits preadipocyte differentiation, while membrane DLK1 represses preadipocyte proliferation. DLK1 is also associated with tumor invasion.
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