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AdipoGen Life Sciences
DLK1 (mouse):Fc (human) (rec.)
290
CHF
CHF 290.00
In stock
AG-40A-0107Y-C01010 µgCHF 290.00
AG-40A-0107Y-30103 x 10 µgCHF 575.00
Replaces AG-40A-0107
Figure 1: Induction of Sox-9 with the treatment of DLK1 (mouse):Fc (human) (rec.) (Prod. No. AG-40A-0107Y).
Method: A mouse preadipocyte cell line, 3T3L-1, was stimulated with 5μg/ml of mouse DLK1:Fc as shown in indicated time points and each cell lysate was prepared and subjected to western blot by using an anti-Sox-9 and anti-GAPDH antibody.
Method: A mouse preadipocyte cell line, 3T3L-1, was stimulated with 5μg/ml of mouse DLK1:Fc as shown in indicated time points and each cell lysate was prepared and subjected to western blot by using an anti-Sox-9 and anti-GAPDH antibody.
Figure 2: Adipogenesis inhibition. Macroscopic pictures.
Figure 3: Adipogenesis inhibition of 3T3L-1 cells using DLK1 (mouse):Fc (human) (rec.) (Prod. No. AG-40A-0107Y).
Method: 3T3L-1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin and glutamine. For differentiation of 3T3L-1 cells, they were cultured in adipogenic medium which was growth medium supplemented with 1μM dexamethasone, 0.5mM IBMX, 10μg/m insulin (day 0). Medium was changed every 3 days. Staining with Oil Red O was typically performed on day 7. Cells were washed twice with PBS, fixed with 3.7% formalin and stained with 0.5% filtered Oil Red O in propylene glycol. Mouse DLK1:Fc (5μg/ml) dissolved in DPBS was added to the differentiation medium. Plates were then used to differentiate 3T3L-1 cells.
Method: 3T3L-1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin and glutamine. For differentiation of 3T3L-1 cells, they were cultured in adipogenic medium which was growth medium supplemented with 1μM dexamethasone, 0.5mM IBMX, 10μg/m insulin (day 0). Medium was changed every 3 days. Staining with Oil Red O was typically performed on day 7. Cells were washed twice with PBS, fixed with 3.7% formalin and stained with 0.5% filtered Oil Red O in propylene glycol. Mouse DLK1:Fc (5μg/ml) dissolved in DPBS was added to the differentiation medium. Plates were then used to differentiate 3T3L-1 cells.
| Product Details | |
|---|---|
| Synonyms | Protein Delta Homolog 1; Fetal Antigen 1; Preadipocyte Factor 1; Pref-1; FA1 |
| Product Type | Protein |
| Properties | |
| Source/Host | HEK 293 cells |
| Sequence |
Signal peptide and extracellular domain of mouse DLK1 (aa 1-305) are fused at the C-terminus to the Fc portion of human IgG1. |
| Crossreactivity | Mouse |
| Biological Activity |
Inhibits adipogenesis of 3T3L-1 cells. Induces Sox-9 in 3T3L-1 cells. |
| MW | ~75kDa (SDS-PAGE) |
| Purity | ≥95% (SDS-PAGE) |
| Endotoxin Content | <0.1EU/μg purified protein (LAL test; Lonza). |
| Concentration | 0.1mg/ml after reconstitution. |
| Reconstitution | Reconstitute with 100μl sterile water. |
| Formulation | Lyophilized. Contains PBS + 0.02% CHAPS. |
| Protein Negative Control | |
| Other Product Data |
UniProt link Q09163: DLK (mouse) |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
The transmembrane and secreted protein delta-like 1 homolog (DLK1; Protein delta homolog 1; Preadipocyte factor 1; Pref-1) belongs to the EGF-like family. It can be cleaved by a tumor necrosis factor-α converting enzyme (TACE) to release a soluble form. DLK1 plays important roles in regulating cell differentiation such as adipogenesis and osteogenesis. Membrane-bound DLK1 promotes hypertrophic myotube formation, whereas soluble DLK1 inhibits myotube formation. The soluble form of DLK1 inhibits preadipocyte differentiation, while membrane DLK1 represses preadipocyte proliferation. DLK1 is also associated with tumor invasion.
Product References
- Genes with physiological roles in callipyge muscle hypertrophy: H. Yu; Purde University Diss. Paper 10 (2013)
- Preadipocyte factor 1 induces pancreatic ductal cell differentiation into insulin-producing cells: M. Rhee, et al.; Sci. Rep. 6, 23960 (2016)
- Identification of genes directly responding to DLK1 signaling in Callipyge sheep: H. Yu, et al.; BMC Genomics 19, 283 (2018)
- Preadipocyte factor 1 regulates adipose tissue browning via TNF-α-converting enzyme-mediated cleavage: M. Rhee, et al.; Metabolism 101, 153977 (2019)
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