anti-MPC-2, mAb (JCM-1)
|Synonyms||Mitochondrial Pyruvate Carrier 2; Brain Protein 44|
|Product Type||Monoclonal Antibody|
|Source/Host||Purified from concentrated hybridoma tissue culture supernatant.|
|Immunogen/Antigen||Peptide coupled to KLH.|
Western Blot: (1µg/ml)
Immunocytochemistry: (1:400) (see online protocol)
Recognizes endogenous human and mouse MPC-2.
|Purity Detail||Protein G-affinity purified.|
|Formulation||Liquid. In PBS containing 25% glycerol and 0.02% sodium azide.|
|Isotype Negative Control|
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
|Use/Stability||Stable for at least 1 year after receipt when stored at -20°C.|
|Product Specification Sheet|
Pyruvate is the end-product of glycolysis, a major substrate for oxidative metabolism, and a branching point for glucose, lactate, fatty acid and amino acid synthesis. The mitochondrial enzymes that metabolize pyruvate are physically separated from cytosolic pyruvate pools and rely on a membrane transport system to shuttle pyruvate across the impermeable inner mitochondrial membrane (IMM). Two proteins, mitochondrial pyruvate carriers MPC-1 and MPC-2, form a heterooligomeric complex in the IMM to facilitate pyruvate transport. This step is required for mitochondrial pyruvate oxidation and carboxylation-critical reactions in intermediary metabolism that are dysregulated in several common metabolic diseases.
- Monitoring mitochondrial pyruvate carrier activity in real time using a BRET-based biosensor: Investigation of the Warburg Effect: V. Compan, et al.; Mol. Cell 59, 491 (2015)
- MPC1-like Is a Placental Mammal-specific Mitochondrial Pyruvate Carrier Subunit Expressed in Postmeiotic Male Germ Cells: B. Vanderperre, et al.; J. Biol. Chem. 291, 16448 (2016)