AdipoGen Life Sciences

anti-TLR4 (human), mAb (ABM19C4)

CHF 390.00
In stock
AG-20T-0303-C100100 µgCHF 390.00
More Information
Product Details
Synonyms Toll-like Receptor 4; TOLL; hToll; CD284; ARMD10
Product Type Monoclonal Antibody
Properties
Clone ABM19C4
Isotype Mouse IgG2bκ
Immunogen/Antigen Recombinant human TLR4 protein (aa 45-320).
Application

Immunohistochemistry: (5μg/ml; paraffin sections)
Flow Cytometry: For FACS use conjugated versions of AG-20T-0303.
Western Blot: (2-4μg/ml)
Optimal conditions must be determined individually for each application.

Crossreactivity Human
Specificity

Recognizes human TLR4.

Purity Protein G purified.
Concentration 0.5mg/ml
Formulation Liquid. In PBS containing 0.05% BSA and 0.05% sodium azide.
Isotype Negative Control

Mouse IgG2b Isotype Control

Accession Number O00206
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Stable for at least 6 months after receipt when stored at +4°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

TLR4 (Toll-like Receptor 4) is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. It was the first TLR identified TLR in mammals and most studied member of the TLR family. TLR4 recognizes the active lipid A component of lipopolysaccharide (LPS) from Gram-negative bacteria in conjunction with LY96 (also referred to MD-2), CD14 and LPS-binding protein (LBP) and consequently mediates the innate immune response to bacterial lipopolysaccharide (LPS). Additionally, it is also involved in LPS-independent responses triggered by endogenous DMAPs, such as heat shock proteins (HSPs) or HMGB1. TLR4 signals via MYD88, TIRAP and TRAF6, leading to NF-κB activation, cytokine secretion and the inflammatory response in various cell types. In complex with TLR6, it was shown to promote sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-β 42. TLR4-mediated inflammation, triggered by exogenous or endogenous ligands, is involved in several acute and chronic diseases, having a pivotal role as amplifier of the inflammatory response. The potency with which this TLR activates inflammatory pathways makes it an ideal target for therapeutic intervention and adjuvant development. A broad range of clinical studies have examined the adjuvant activity of TLR4 activators in vaccines against infectious and tumor antigens.

© 2017 Adipogen Life Sciences. Pictures: © 2012 Martin Oeggerli. All Rights Reserved.