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AdipoGen Life Sciences
Kynurenine Aminotransferase II (human) (rec.) (His)
590
CHF
CHF 590.00
In stock
AG-40T-0102-C05050 µgCHF 590.00
| Product Details | |
|---|---|
| Synonyms | hKAT II; KAT2; α-Aminoadipate Aminotransferase (Mitochondrial); AADAT; EC 2.6.1.7 |
| Product Type | Protein |
| Properties | |
| Source/Host | E. coli |
| Sequence | Human full-length KAT II is fused at the N-terminus to a His-tag. |
| Crossreactivity | Human |
| Application | Can be used to test potential kynurenic acid synthesis inhibitors by performing either in vitro end point assays followed by HPLC-UV (Lit. 2) or continuous spectrophotometric analysis (Lit. 3). |
| MW | ~49.8kDa |
| Purity | ≥97% (SDS-PAGE) |
| Endotoxin Content | N/A |
| Concentration | 0.5mg/ml (Lot specific) |
| Formulation | Liquid. In 50 mM phosphate buffer pH 8.0, containing 50mM sodium chloride and 40μM pyridoxal 5'-phosphate (PLP). |
| Other Product Data | NCBI link NP_872603: AADAT (human) |
| Shipping and Handling | |
| Shipping | DRY ICE |
| Short Term Storage | -20°C |
| Long Term Storage | -80°C |
| Handling Advice |
After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 6 months after receipt when stored at -80°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Kynurenine aminotransferases (KATs) are pyridoxal-5’-phosphate-dependent enzymes that catalyze the conversion of L-kynurenine into kynurenic acid, a neuroactive metabolite whose unbalancing is associated with a number of brain disorders. Biochemical and structural investigations revealed that L-kynurenine (L-KYN) recognition by hKAT II is achieved by exploiting structural features that are peculiar of this isoform, thus offering the possibility to select/design inhibitor molecules specifically targeting hKAT II to be used as modulators of kynurenic acid synthesis in the CNS. hKAT II is one of the aminotransferases involved in the pyridoxal 5'-phosphate (PLP)-dependent irreversible transamination of L-kynurenine (L-KYN) to kynurenic acid (KYNA) in the central nervous system. When assayed in vitro the protein also displays β-elimination activity.
Product References
- Crystal Structure of Human Kynurenine Aminotransferase II, a Drug Target for the Treatment of Schizophrenia; F. Rossi, et al.; J. Biol. Chem. 283, 3559 (2008)
- Biochemical and Structural Investigations on Kynurenine Aminotransferase II: An Example of Conformation-Driven Species-Specific Inhibition?; V. Casazza, et al.; Curr. Top. Med. Chem. 11, 148 (2011)
- Human kynurenine aminotransferase II - reactivity with substrates and inhibitors; E. Passera, et al.; FEBS J. 278, 1882 (2011)
- A covalent small molecule inhibitor of glutamate-oxaloacetate transaminase 1 impairs pancreatic cancer growth: T. Yoshida, et al.; BBRC 522, 633 (2019)