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Cayman
Myeloperoxidase Chlorination Fluorometric Assay Kit
391
CHF
CHF 391.00
In stock
CAY-10006438-2192 wellsCHF 391.00
| Product Details | |
|---|---|
| Synonyms | MPO |
| Product Type | Kit |
| Properties | |
| Application Set | Compound Screening |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| Declaration | Manufactured by Cayman Chemicals. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Long Term Storage | +4°C |
| Documents | |
| Manual | No |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
Cayman's Myeloperoxidase Chlorination Fluorometric Assay provides a convenient fluorescence-based method for detecting the MPO chlorination activity in both crude cell lysates and purified enzyme preparations. The assay utilizes non-fluorescent 2-[6-(4-aminophenoxy)-3-oxo-3H-xanthen-9-yl]-benzoic acid (APF), which is selectively cleaved by hypochlorite (-OCl) to yield the highly fluorescent compound fluorescein. Fluorescein fluorescence is analyzed with an excitation wavelength of 480-495 nm and an emission wavelength of 515-525 nm. The kit includes a MPO-specific inhibitor for distinguishing MPO activity from MPO-independent fluorescence.