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Cayman
NF-κB (human p50) Transcription Factor Assay Kit
759
CHF
CHF 759.00
In stock
CAY-10006912-9696 wellsCHF 759.00
Product Details | |
---|---|
Product Type | Kit |
Properties | |
Application Set | Compound Screening |
Other Product Data |
Click here for Original Manufacturer Product Datasheet |
Declaration | Manufactured by Cayman Chemicals. |
Shipping and Handling | |
Shipping | DRY ICE |
Long Term Storage | -80°C |
Documents | |
Manual | No |
MSDS | Inquire |
Product Specification Sheet | |
Datasheet | Download PDF |
Description
Cayman's NF-κB (human p50) Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the NF-κB response element is immobilized to the wells of a 96-well plate. NF-κB contained in a nuclear extract, binds specifically to the NF-κB response element. NF-κB (p50) is detected by addition of specific primary antibody directed against NF-κB (p50). A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. The Cayman Chemical NF-κB (human p50) Transcription Factor Assay detects human NF-κB (p50). It will not cross-react with NF-κB (p65).