Cookie Policy: This site uses cookies to improve your experience. You can find out more about our use of cookies in our Privacy Policy. By continuing to browse this site you agree to our use of cookies.
Cayman
PAD4 Inhibitor Screening Assay Kit (AMC)
759
CHF
CHF 759.00
In stock
CAY-701320-9696 wellsCHF 759.00
Product Details | |
---|---|
Synonyms | PADI4; PADI5; PDI5; Peptidylarginine Deiminase 4; Protein Arginine Deiminase 4 |
Product Type | Kit |
Properties | |
Application Set | Compound Screening |
Other Product Data |
Click here for Original Manufacturer Product Datasheet |
Declaration | Manufactured by Cayman Chemicals. |
Shipping and Handling | |
Shipping | DRY ICE |
Long Term Storage | -80°C |
Documents | |
Manual | No |
MSDS | Inquire |
Product Specification Sheet | |
Datasheet | Download PDF |
Description
Cayman's PAD4 Inhibitor Screening Assay Kit (AMC) provides a convenient method for screening human PAD4 inhibitors. This assay utilizes a fluorescent substrate (Z-Arg-AMC) consisting of an arginine residue, a carboxybenzyl group, and a fluorophore (7-amino-4-methylcoumarin, AMC). Acylation of AMC onto the arginine residue masks the fluorescence of the fluorophore. In the absence of PAD4, the substrate remains unaltered, allowing the developer to release free AMC. In the presence of PAD4, the arginine of the substrate is citrullinated, and while the reaction is quenched by the addition of developer, free AMC is not released. Fluorescence is analyzed with an excitation wavelength of 355-365 nm and an emission wavelength of 445-455 nm. The fluorescent signal is inversely proportional to the amount of citrullination by PAD4.