anti-GPx8 (human), pAb (IN103)
|Synonyms||Probable Glutathione Peroxidase 8; GSHPx-8|
|Product Type||Polyclonal Antibody|
|Immunogen/Antigen||Recombinant GPx8 (human) (aa 38-209) fused to a GST-tag.|
Western Blot: (1:1'000)
Recognizes human GPx8.
|Purity Detail||Protein-A affinity purified.|
|Formulation||Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.|
|Isotype Negative Control|
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
|Use/Stability||Stable for at least 1 year after receipt when stored at -20°C.|
|Product Specification Sheet|
GPxs are glutathione peroxidases involved in balancing the H2O2 homeostasis in signaling cascades. GPxs have been known to catalyze the reduction of H2O2 or organic hydroperoxides to water or the corresponding alcohols, respectively, typically using glutathione (GSH) as reductant. GPx8 (probable glutathione peroxidase 8) is a 209-aa, 24kDa protein with an N-terminal cytosolic tip, a predicted transmembrane segment and a catalytic domain located in the endoplasmic reticulum (ER) lumen. GPx8 is a CysGPxs with low glutathione peroxidase activity. A recent study identified GPx8 as a cellular substrate of the hepatitis C virus NS3-4A protease. GPx8 cleavage by NS3-4A occurs at Cys11, removing the cytosolic tip of GPx8 and was observed in different experimental systems as well as in liver biopsies from patients with chronic hepatitis C.
- Quantitative proteomics identifies the membrane-associated peroxidase GPx8 as a cellular substrate of the hepatitis C virus NS3-4A protease: K. Morikawa, et al.; Hepatology 59, 423 (2014)
- Regulation of Calcium Fluxes by GPX8, a Type-II Transmembrane Peroxidase Enriched at the MitochondriaAssociated Endoplasmic Reticulum Membrane: E.D. Yoboue, et al.; Antioxid. Redox. Signal 27, 583 (2017)
- Nrf2 signaling links ER oxidative protein folding and calcium homeostasis in health and disease: V. Granatiero, et al.; Life Sci. Alliance 2, e201900563 (2019)